Cow Milk and Intestinal Epithelial Cell-derived Extracellular Vesicles as Systems for Enhancing Oral Drug Delivery

Pharmaceutics
Greta CarobolanteDriton Vllasaliu

Abstract

Ingestion is the preferred way for drug administration. However, many drugs have poor oral bioavailability, warranting the use of injections. Extracellular vesicles (EVs) from cow milk have shown potential utility in improving oral drug bioavailability. However, EVs produced by intestinal epithelial cells have not been investigated for this application. We compared the capacity of cow milk EVs and intestinal epithelial cell-derived counterparts to enhance oral drug bioavailability. EVs were isolated, fluorescently labelled, and loaded with curcumin (CUR) as a model poorly absorbable drug. These were then characterised before testing in an intestinal model (Caco-2). Epithelial cell-derived EVs showed notably higher cell uptake compared to cow milk EVs. Cell uptake was significantly higher in differentiated compared to undifferentiated cells for both types of EVs. While both milk- and cell-derived EVs improved the cell uptake and intestinal permeability of CUR (confirming oral drug bioavailability enhancement potential), epithelial cell EVs demonstrated a superior effect.

References

May 23, 2001·Advanced Drug Delivery Reviews·K KhanvilkarD R Flanagan
Nov 15, 2007·Molecular Pharmaceutics·Preetha AnandBharat B Aggarwal
Apr 23, 2010·Pharmaceutical Research·Gert FrickerChristel Müller-Goymann
Nov 26, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Yukihiro AkaoTomoki Naoe
Nov 5, 2013·Clinical Biochemistry·Kadri RekkerMaire Peters
Nov 29, 2013·Science Translational Medicine·Eric M PridgenOmid C Farokhzad
Dec 20, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Tyson SmythThomas J Anchordoquy
May 17, 2015·Journal of Extracellular Vesicles·María Yáñez-MóOlivier De Wever
Nov 26, 2015·Cancer Letters·Radha MunagalaRamesh C Gupta
Mar 18, 2016·American Journal of Physiology. Cell Physiology·Rio Jati KusumaJanos Zempleni
Apr 14, 2016·Nanomedicine·Thanh Xuan NguyenQun Wang
Nov 3, 2016·The Journal of Nutrition·Abderrahim BenmoussaPatrick Provost
Jan 4, 2017·Matrix Biology : Journal of the International Society for Matrix Biology·Ambra PozziRenato V Iozzo
Mar 17, 2017·Nanomedicine : Nanotechnology, Biology, and Medicine·Ashish K AgrawalRamesh C Gupta
May 4, 2017·Applied Biochemistry and Biotechnology·Monika VashishtDheer Singh
Jul 9, 2017·Molecular Nutrition & Food Research·Yalin LiaoBo Lönnerdal
Jul 12, 2017·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Joanna L BirdAlan D Goddard
Oct 20, 2017·The AAPS Journal·Farrukh AqilRamesh Gupta
Nov 24, 2018·Journal of Pharmaceutical Sciences·Jamie L BetkerThomas J Anchordoquy

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Citations

Sep 17, 2020·International Journal of Molecular Sciences·Sergey SedykhGeorgy Nevinsky
Oct 8, 2020·International Journal of Molecular Sciences·Ki-Uk KimHyeyoung Min
Dec 29, 2020·Biomaterials Science·Matthew R WarrenAmbika G Bajpayee
Mar 20, 2021·Frontiers in Cell and Developmental Biology·Hang SongJinghui Wang
Aug 20, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Laura CabezaJose Prados
May 1, 2021·Pharmaceutics·Driton Vllasaliu
May 21, 2021·Nanomedicine·Xin-Hui CaoWei Zhang
Jun 3, 2021·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Josepha RoerigMichaela Schulz-Siegmund
Jun 3, 2021·International Journal of Molecular Sciences·Andrew E MasseyBilal B Hafeez
Jun 7, 2021·Stem Cell Research & Therapy·Ruijie ZengHao Chen
Sep 17, 2021·International Journal of Pharmaceutics·Shilpa Lekhraj Peswani SajnaniDriton Vllasaliu

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Methods Mentioned

BETA
Assay
dynamic light scattering
protein assay
size exclusion chromatography

Software Mentioned

GraphPad Prism

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