COX-1 and COX-2 contribute differentially to the LPS-induced release of PGE2 and TxA2 in liver macrophages

Prostaglandins & Other Lipid Mediators
Yevgeniya BezuglaPeter Dieter

Abstract

LPS induces an immediate release of thromboxane TxA2 and a delayed release of PGE2. Dexamethasone suppresses the LPS-induced release of TxA2 and PGE2. In the first 8 h after LPS addition, the specific COX-2 inhibitor SC236 inhibits the PGE2 and TxA2 release by about 80% and 20%, whereas the release of PGE2 and TxA2 between 8 and 24 h is inhibited by about 40% and 35%, respectively. Resident liver macrophages express substantial amounts of COX-1, TxAS, cPGES and mPGES-2, small amounts of COX-2 but almost no detectable amounts of mPGES-1. LPS induces an increase of COX-2 and mPGES-1, but does not change COX-1, cPGES, mPGES-2 and TxAS at protein level. Dexamethasone suppresses almost completely the LPS-induced effects on COX-2 and mPGES-1. It is concluded that (1) COX-1 and COX-2 are involved in the LPS-induced synthesis of TxA2 and PGE2; (2) TxA2 release is catalyzed at early time-points by the combined action of COX-1 and TxAs, whereas at later time points the newly expressed COX-2 couples to TxAS and contributes to the TxA2 release; (3) PGE2 release within the first 8 h is predominantly catalyzed by COX-2, whereas at later time-points COX-1 couples to the newly expressed mPGES-1 and contributes to the PGE2 release.

References

Sep 11, 1990·European Journal of Biochemistry·K Decker
Oct 15, 1988·European Journal of Biochemistry·P DieterK Decker
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·P J JakobssonB Samuelsson
Feb 15, 2000·American Journal of Respiratory and Critical Care Medicine·E A Dennis
Aug 31, 2000·Annual Review of Biochemistry·W L SmithR M Garavito
Oct 12, 2000·Biochemical and Biophysical Research Communications·P DieterS Kamionka
Feb 28, 2002·Biochemical and Biophysical Research Communications·Naomi TanikawaKikuko Watanabe
Nov 16, 2002·Prostaglandins & Other Lipid Mediators·Makoto MurakamiIchiro Kudo
Apr 4, 2003·The Journal of Biological Chemistry·Staffan ThorénPer-Johan Jakobsson
Nov 26, 2003·Progress in Lipid Research·Makoto Murakami, Ichiro Kudo

❮ Previous
Next ❯

Citations

Sep 15, 2010·PloS One·Karen L DenzlerJeffrey O Langland
May 8, 2007·Proceedings of the National Academy of Sciences of the United States of America·Manuel Sanchez-Alavez, Tamas Bartfai
Jun 17, 2009·Journal of Cardiovascular Pharmacology·Laura Lada-MoldovanGuy Rousseau
Feb 2, 2010·Prostaglandins & Other Lipid Mediators·Reiner KunzePeter Dieter
Nov 13, 2007·Experimental and Molecular Pathology·Li C ChenDebra L Laskin
May 16, 2009·The Laryngoscope·Vlad C SandulachePatricia A Hebda
Dec 23, 2011·Environmental Toxicology and Chemistry·Carlie A LaLoneGerald T Ankley
Dec 15, 2010·Toxicon : Official Journal of the International Society on Toxinology·Vanessa MoreiraCatarina Teixeira
Jul 27, 2010·Progress in Lipid Research·Melanie J Stables, Derek W Gilroy
Jul 31, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Katrin BirkenmeierStephan B Felix
Aug 1, 2014·American Journal of Physiology. Cell Physiology·Hercules A da Silva-SouzaEliana Scemes
Apr 23, 2016·American Journal of Physiology. Gastrointestinal and Liver Physiology·Lu YaoTong Wu
Sep 6, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xinzhi LiColin D Funk
Dec 15, 2006·World Journal of Gastroenterology : WJG·George KoliosElias Kouroumalis
Nov 29, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Poonam KothariDomenick J Falcone
Jan 10, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·K M Faisal KhanDomenick J Falcone
Feb 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Matthew D WoolardJeffrey A Frelinger
Sep 8, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Ji MaTao Zhang
Nov 14, 2016·Microbiology Spectrum·Melanie Bennett, Derek W Gilroy
Oct 30, 2021·Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis·Faruk Aydınyılmazİbrahim Halil Tanboğa

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved