COX-2, CB2 and P2X7-immunoreactivities are increased in activated microglial cells/macrophages of multiple sclerosis and amyotrophic lateral sclerosis spinal cord

BMC Neurology
Yiangos YiangouPraveen Anand

Abstract

While multiple sclerosis (MS) and amyotrophic lateral sclerosis (ALS) are primarily inflammatory and degenerative disorders respectively, there is increasing evidence for shared cellular mechanisms that may affect disease progression, particularly glial responses. Cyclooxygenase 2 (COX-2) inhibition prolongs survival and cannabinoids ameliorate progression of clinical disease in animal models of ALS and MS respectively, but the mechanism is uncertain. Therefore, three key molecules known to be expressed in activated microglial cells/macrophages, COX-2, CB2 and P2X7, which plays a role in inflammatory cascades, were studied in MS and ALS post-mortem human spinal cord. Frozen human post mortem spinal cord specimens, controls (n = 12), ALS (n = 9) and MS (n = 19), were available for study by immunocytochemistry and Western blotting, using specific antibodies to COX-2, CB2 and P2X7, and markers of microglial cells/macrophages (CD 68, ferritin). In addition, autoradiography for peripheral benzodiazepine binding sites was performed on some spinal cord sections using [3H] (R)-PK11195, a marker of activated microglial cells/macrophages. Results of immunostaining and Western blotting were quantified by computerized image and optical den...Continue Reading

References

Nov 1, 1994·Immunopharmacology·I WirguinT Brenner
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·K SeibertP Isakson
Mar 3, 1994·The New England Journal of Medicine·G BensimonV Meininger
Sep 13, 1996·Biochemical Pharmacology·R G CoffeyS Pross
Nov 22, 1996·The Journal of Biological Chemistry·D G PerregauxC A Gabel
Nov 19, 1997·Neuropharmacology·G ColloG Buell
Jan 1, 1997·Advances in Experimental Medicine and Biology·K SeibertP Isakson
Jan 1, 1997·Advances in Experimental Medicine and Biology·M Goppelt-Struebe, F Beiche
Jun 18, 1998·Annals of Neurology·T SairanenP J Lindsberg
Aug 26, 1998·Immunology Today·T W KleinH Friedman
Dec 9, 1998·The American Journal of Physiology·D G Perregaux, C A Gabel
Jan 1, 1999·Journal of Neurotrauma·D K ResnickD W Marion
Jan 19, 1999·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·F BeicheM Goppelt-Struebe
May 1, 1999·Critical Reviews in Neurobiology·M K O'Banion
Oct 4, 2000·The Journal of Biological Chemistry·M SolleC A Gabel
May 9, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·C Verderio, M Matteoli
Sep 29, 2001·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Y YiangouP Anand
Nov 6, 2001·Journal of Neuroimmunology·B BadieJ Vorpahl
Jan 5, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Carola G SchipkeHelmut Kettenmann
Apr 25, 2002·Science's STKE : Signal Transduction Knowledge Environment·R MechoulamE Shohami
May 31, 2002·Pharmacological Reviews·A C HowlettR G Pertwee
Nov 5, 2002·The Journal of Biological Chemistry·Silvia CocoClaudia Verderio
Jan 29, 2003·Current Opinion in Pharmacology·T Philip MalanFrank Porreca
Feb 15, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·P N PomplG M Pasinetti
Apr 17, 2003·The Journal of Clinical Investigation·J Ludovic Croxford, Stephen D Miller
Jul 24, 2003·Brain : a Journal of Neurology·Gareth PryceDavid Baker
Aug 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Mohab M IbrahimT Philip Malan

❮ Previous
Next ❯

Citations

Jun 17, 2010·Journal of Neural Transmission·Ana G BarbeitoSéverine Boillée
May 5, 2012·Journal of Neural Transmission·Joanna TarasiukPiotr Lewczuk
Dec 3, 2013·Inflammation·Domenico NuzzoMarta Di Carlo
Apr 12, 2008·Purinergic Signalling·H FrankeU Krügel
Feb 14, 2009·Purinergic Signalling·Robson Coutinho-SilvaDavid M Ojcius
Feb 12, 2009·Purinergic Signalling·Maria Luisa Cotrina, Maiken Nedergaard
Jul 28, 2011·Purinergic Signalling·Marek CieślakMichał Komoszyński
Mar 9, 2013·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Slava Rom, Yuri Persidsky
Oct 24, 2007·Molecular Neurobiology·Roger G Pertwee
Oct 24, 2007·Molecular Neurobiology·Maulik D JhaveriVictoria Chapman
Oct 1, 2009·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Elizabeth J Rahn, Andrea G Hohmann
Jan 21, 2009·Expert Reviews in Molecular Medicine·Guy A Cabral, LaToya Griffin-Thomas
Jan 9, 2013·Proceedings of the National Academy of Sciences of the United States of America·Jacqueline L BlankmanBenjamin F Cravatt
Dec 3, 2009·Antioxidants & Redox Signaling·Sergio Rosales-CorralGabriela Lopez-Armas
Sep 29, 2009·Cerebral Cortex·Susanna AmadioCinzia Volonté
Aug 27, 2009·Journal of Neuroinflammation·Jessica M CrainJyoti J Watters
Jun 14, 2012·PloS One·Yoshifumi IwamaruHiroshi Kitani
Dec 15, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jill BouchardPaul J Muchowski
Mar 24, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Servio H RamirezYuri Persidsky
Feb 1, 2013·Autoimmune Diseases·Makoto Inoue, Mari L Shinohara
Nov 13, 2013·Cellular and Molecular Life Sciences : CMLS·Hjalte Holm AndersenTorben Moos
Mar 17, 2010·Expert Opinion on Therapeutic Targets·Javier Fernández-RuizEva de Lago
Nov 17, 2015·Expert Opinion on Therapeutic Targets·Emiliano Peña-AltamiraBarbara Monti
Nov 20, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sabrina GiacoppoEmanuela Mazzon
May 29, 2012·Amyotrophic Lateral Sclerosis : Official Publication of the World Federation of Neurology Research Group on Motor Neuron Diseases·UNKNOWN ALSUntangled Group
Nov 16, 2013·Amyotrophic Lateral Sclerosis & Frontotemporal Degeneration·Fei SongJeffrey A Loeb
Jan 5, 2016·The Journal of Organic Chemistry·Piotr SzcześniakSebastian Stecko
Dec 5, 2015·Nature Reviews. Drug Discovery·Knut BiberMarco Prinz
Sep 4, 2015·Molecular Neurobiology·Tatsurou YagamiYasuhiro Yamamoto
Jan 23, 2008·Expert Opinion on Therapeutic Targets·Fabian DocagneCarmen Guaza
Aug 15, 2014·Journal of Neural Transmission·Pascal F DurrenbergerRichard Reynolds

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Methods Mentioned

BETA
transgenic

Software Mentioned

SeeScan
GraphPad Prism

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