COX-2 mRNA expression in esophageal squamous cell carcinoma (ESCC) and effect by NSAID

Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus
X LiuZ L Yu

Abstract

To investigate cyclooxygenase-2 (COX-2) mRNA expression in human esophageal squamous cell carcinoma and the effect of a non-steroidal anti-inflammatory drug (NSAID) on it, in order to explore the mechanism of COX-2 in esophageal squamous cell carcinoma (ESCC) carcinogenesis and the ability of NSAID to prevent or treat ESCC. Frozen specimens of human ESCC and adjacent normal esophageal squamous epithelium pairs (n = 22) were examined for COX-2 mRNA expression by reverse-transcription polymerase chain reaction (RT-PCR). After incubation with aspirin (a non-selective COX inhibitor) or Nimesulide (a selective COX-2 inhibitor), the proliferation status of two human esophageal squamous cancer cell lines, EC-9706 and EC-109, was quantified by 3-(4,5-dimethyl-thiazol-2yl)-2,5-diphenyltetrazolium bromide assay. The expression of COX-2 mRNA in these cells was detected by RT-PCR. COX-2 mRNA was expressed in 12 of 22 (54.5%) ESCC tissue samples, but it was undetectable in all the specimens of adjacent normal esophageal squamous epithelium COX-2 mRNA expression. Both aspirin (5-20 mmol/L) and Nimesulide (0.1-0.8 mmol/L) inhibited EC-9706 cell line proliferation and suppressed its COX-2 mRNA expression dose-dependently. However, only aspirin...Continue Reading

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Citations

Jul 3, 2010·Journal of Gastroenterology and Hepatology·Jing DongHongbing Shen
Jan 5, 2014·BMC Gastroenterology·Hennie M J RoelofsWilbert H M Peters
Jul 25, 2009·World Journal of Gastroenterology : WJG·Jón O KristinssonWilbert H M Peters
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Feb 10, 2011·The Tohoku Journal of Experimental Medicine·Yuan LiangRong Wu

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