Cox2p of yeast cytochrome oxidase assembles as a stand-alone subunit with the Cox1p and Cox3p modules

The Journal of Biological Chemistry
Leticia Veloso R FrancoAlexander Tzagoloff

Abstract

Cytochrome oxidase (COX) is a hetero-oligomeric complex of the mitochondrial inner membrane that reduces molecular oxygen to water, a reaction coupled to proton transfer from the mitochondrial matrix to the intermembrane space. In the yeast Saccharomyces cerevisiae, COX is composed of 11-13 different polypeptide subunits. Here, using pulse labeling of mitochondrial gene products in isolated yeast mitochondria, combined with purification of tagged COX subunits and ancillary factors, we studied the Cox2p assembly intermediates. Analysis of radiolabeled Cox2p obtained in pulldown assays by native gel electrophoresis revealed the existence of several assembly intermediates, the largest of which had an estimated mass of 450-550 kDa. None of the other known subunits of COX were present in these Cox2p intermediates. This was also true for the several ancillary factors having still undefined functions in COX assembly. In agreement with earlier evidence, Cox18p and Cox20p, previously shown to be involved in processing and in membrane insertion of the Cox2p precursor, were found to be associated with the two largest Cox2p intermediates. A small fraction of the Cox2p module contained Sco1p and Coa6p, which have been implicated in metalati...Continue Reading

References

Oct 1, 1990·Molecular & General Genetics : MGG·E DecosterG Faye
Jan 1, 1983·Methods in Enzymology·R J Rothstein
Sep 8, 2001·The Journal of Biological Chemistry·T NittisD R Winge
Nov 26, 2002·The Journal of Biological Chemistry·Christopher G CarlsonD Moira Glerum
Nov 1, 2003·The EMBO Journal·Xochitl Perez-MartinezThomas D Fox
Aug 13, 2004·The EMBO Journal·Antoni BarrientosAlexander Tzagoloff
Jul 11, 2006·Biochimica Et Biophysica Acta·Shinya YoshikawaHideo Shimada
Apr 5, 2007·Nature Protocols·Ilka WittigHermann Schägger
Oct 28, 2009·Molecular and Cellular Biology·Flavia FontanesiAntoni Barrientos
Sep 30, 2010·The Journal of Cell Biology·David U MickPeter Rehling
Dec 24, 2010·Nature Reviews. Molecular Cell Biology·David U MickPeter Rehling
Jan 27, 2011·The EMBO Journal·Malgorzata RakAlexander Tzagoloff
Oct 1, 2011·Biochimica Et Biophysica Acta·Ileana C SotoAntoni Barrientos
May 5, 2012·Biochimica Et Biophysica Acta·Hyung J KimDennis R Winge
Dec 26, 2012·Molecular Biology of the Cell·Gavin P McStayAlexander Tzagoloff
Jul 31, 2013·The Journal of Biological Chemistry·Gavin P McStayAlexander Tzagoloff
Aug 28, 2013·Molecular and Cellular Biology·Bettina BarethPeter Rehling
Oct 24, 2014·Human Mutation·Fabian BaertlingRichard J T Rodenburg
Dec 17, 2015·Human Molecular Genetics·Alok GhoshVishal M Gohil
Mar 2, 2016·The Journal of Biological Chemistry·Juan Pablo MayorgaXochitl Pérez-Martínez
Apr 17, 2016·Biochimica Et Biophysica Acta·Maria LevchenkoMarkus Deckers
Apr 20, 2016·Biochimica Et Biophysica Acta·Valentina StreckerIlka Wittig
Dec 22, 2016·The Journal of Biological Chemistry·Nicholas G TaylorOleh Khalimonchuk
Aug 20, 2017·The Journal of Biological Chemistry·Chen-Hsien Su, Alexander Tzagoloff

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Citations

Mar 7, 2020·Biological Chemistry·Leticia Veloso Ribeiro FrancoAlexander Tzagoloff
Oct 4, 2020·International Journal of Molecular Sciences·Shane A Watson, Gavin P McStay
Nov 2, 2019·Mitochondrion·Mario H Barros, Gavin P McStay

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