Coxsackievirus-adenovirus receptor expression in ovarian cancer cell lines is associated with increased adenovirus transduction efficiency and transgene expression

Cancer Gene Therapy
Z YouD G Kieback

Abstract

The expression of coxsackievirus-adenovirus receptor (CAR) and the integrins alpha(v)beta3 and alpha(v)beta5 was analyzed quantitatively (flow cytometry) and qualitatively (immunocytochemistry) in five human ovarian cancer cell lines (PEO1, PEO4, PEO14, SKOV-3, and OVCAR-3) and three control cell lines (293, HeLa, and CHO-K1). The transduction efficiencies were evaluated by adv/rsv-beta-Gal transduction followed by X-gal staining. The effects of 17beta-estradiol on cell growth, CAR and integrins alpha(v)beta3/5 expression, adenovirus transduction efficiency, and cell-killing efficacy of adv/rsv-tk plus ganciclovir were determined. The levels of CAR, integrin alpha(v)beta3, and integrin alpha(v)beta5 showed great variation between the cell lines. Whereas the expression of CAR appeared to be essential for and positively correlated with adenovirus transduction efficiency, the integrins alpha(v)beta3 and alpha(v)beta5 were not absolutely necessary for adenovirus transduction even though their presence may facilitate transduction. In PEO4 and PEO1 cells, proliferation was stimulated by 17beta-estradiol in a dose-dependent manner. In PEO4 cells, and much less pronounced in PEO1 cells, this was accompanied by an increase in CAR expres...Continue Reading

Citations

Apr 14, 2006·Cancer Immunology, Immunotherapy : CII·Susan J HedleyImre Kovesdi
Jul 12, 2008·Breast Cancer Research and Treatment·Doris AuerAlain G Zeimet
Sep 27, 2003·Journal of Virology·Laura FontanaPaolo Monaci
Aug 15, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·M KrivegaH Van de Velde
Aug 7, 2004·Gynecologic Oncology·Charles A LeathParameshwar J Mahasreshti
Sep 19, 2003·Biochemical and Biophysical Research Communications·Annick LucasGwendal Lazennec
Sep 2, 2015·Nature Reviews. Drug Discovery·Howard L KaufmanAndrew Zloza
Nov 22, 2005·Oncogene·J Michael MathisDavid T Curiel
Feb 14, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Qing YuDon C Rockey
Sep 21, 2020·Cancer Gene Therapy·Rebeca Gonzalez-PastorDavid T Curiel
Sep 12, 2018·International Journal of Molecular Sciences·Manh-Hung DoChaeyong Jung
Feb 18, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Tsuyoshi SasakiToshiyoshi Fujiwara
Jun 29, 2018·Porto Biomedical Journal·Joseph HoareElisabete Carapuça

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