PMID: 8590797Dec 1, 1995Paper

CP60: a microtubule-associated protein that is localized to the centrosome in a cell cycle-specific manner

Molecular Biology of the Cell
D R KelloggB M Alberts

Abstract

DMAP190 is a microtubule-associated protein from Drosophila that is localized to the centrosome. In a previous study, we used affinity chromatography to identify proteins that interact with DMAP190, and identified a 60-kDa protein that we named DMAP60 (Kellogg and Alberts, 1992). Like DMAP190, DMAP60 interacts with microtubules and is localized to the centrosome, and the two proteins associate as part of a multiprotein complex. We now report the cloning and sequencing of the cDNA encoding DMAP60. The amino acid sequence of DMAP60 is not homologous to any protein in the database, although it contains six consensus sites for phosphorylation by cyclin-dependent kinases. As judged by in situ hybridization, the gene for DMAP60 maps to chromosomal region 46A. In agreement with others working on Drosophila centrosomal proteins, we have changed the names for DMAP190 and DMAP60 to CP190 and CP60, respectively, to give these proteins a consistent nomenclature. Antibodies that recognize CP60 reveal that it is localized to the centrosome in a cell cycle-dependent manner. The amount of CP60 at the centrosome is maximal during anaphase and telophase, and then drops dramatically during late telophase or early interphase. This dramatic disappe...Continue Reading

References

Jan 1, 1992·Molecular Biology of the Cell·G BarnesD Botstein
Mar 1, 1992·The Journal of Cell Biology·C H YangM Snyder
Feb 1, 1992·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R Kuriyama, C Nislow
Jan 1, 1991·Annual Review of Cell Biology·L MarshI Herskowitz
Oct 1, 1991·Current Opinion in Genetics & Development·S I Reed
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·I R GibbonsB H Gibbons
Jan 10, 1991·Nature·M GlotzerM W Kirschner
Nov 1, 1991·The Journal of Cell Biology·K A Butner, M W Kirschner
Sep 20, 1988·Journal of Molecular Biology·N H Brown, F C Kafatos
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D D VandreG G Borisy
Nov 15, 1984·Nature·T Mitchison, M Kirschner
Oct 1, 1995·The Journal of Cell Biology·K G Miller, D P Kiehart
Aug 1, 1995·The Journal of Cell Biology·D R KelloggA W Murray
Jan 1, 1994·Annual Review of Biochemistry·D R KelloggB M Alberts
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·S MarcusM Wigler

❮ Previous
Next ❯

Citations

Jul 16, 2008·Biochemistry. Biokhimii︠a︡·I B Alieva, R E Uzbekov
Apr 25, 2008·PLoS Biology·Julian R HughesJames G Wakefield
Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·F GergelyJ W Raff
Jul 14, 1997·The Journal of Cell Biology·R Altman, D Kellogg
Jan 4, 2001·Drug Metabolism Reviews·P M Flatt, J A Pietenpol
Dec 22, 2006·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·William S TurnerLola M Reid
Jan 25, 2007·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·J ChenW O Soboyejo
Feb 21, 2006·Journal of Experimental Zoology. Part A, Comparative Experimental Biology·Hideki IkedaMasayuki Saigusa
May 11, 2010·Advances in Colloid and Interface Science·Kazue Kurihara
Aug 7, 2002·The American Journal of Pathology·Francesco FacchianoMaurizio C Capogrossi
Apr 20, 2001·Trends in Neurosciences·O H PetersenM K Park
Apr 28, 2010·PloS One·Catalina Alfonso-Parra, Keith A Maggert
Jul 10, 2016·Advances in Colloid and Interface Science·Russell J StewartJoshua P Jones
Apr 8, 2006·Medical Hypotheses·Ying Chen, Qingzhong Kong
Jun 24, 1999·Journal of Biomedical Materials Research·X F WalboomersJ A Jansen
Mar 17, 2017·Annual Review of Physical Chemistry·Elnaz AlipourGilbert C Walker
Sep 20, 2001·Memórias do Instituto Oswaldo Cruz·J A GutiérrezL A Murillo
Oct 25, 2014·Genome Research·Oksana MaksimenkoPavel Georgiev
May 12, 2000·Nature Cell Biology·M Wasser, W Chia
Oct 18, 2011·Integrative Biology : Quantitative Biosciences From Nano to Macro·Takuma KanesakiJörg Grosshans
Mar 10, 2000·The American Journal of Gastroenterology·W PolkowskiG J Offerhaus
Sep 7, 1999·Nature·W M ClemonsV Ramakrishnan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Cycle Pathways

Cell cycle is a complex process regulated by several signal transduction pathways and enzymes. Here is the latest research on regulation of cell cycle and cell cycle pathways.

Cell Cycle Control & Proteolysis

Key regulators of cell cycle, including cyclins, cyclin dependent kinase inhibitors, DNA replication factors, are controlled by proteolysis. Discover the latest research on cell cycle control and proteolysis.

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.