CPAP interacts with 14-3-3 in a cell cycle-dependent manner

Biochemical and Biophysical Research Communications
Chiung-Ya ChenTang K Tang

Abstract

We have previously reported that CPAP (Centrosomal Protein 4.1-Associated Protein) carries a novel microtubule-destabilizing motif that may regulate microtubule dynamics at the centrosome. In this study, we searched for conserved sequence motifs in CPAP and identified two classical 14-3-3 binding sites. The interaction between CPAP and 14-3-3 was demonstrated by both yeast two-hybrid and co-immunoprecipitation experiments. Further analyses revealed that the 14-3-3 binding motif is located within the C-terminal domain of CPAP. Alkaline phosphatase treatment disrupted CPAP binding to 14-3-3, suggesting that phosphorylation is required for the interaction. Mutation of serine 1109 to alanine (S1109A) in the 14-3-3 binding motif completely abolished the association of CPAP with 14-3-3. Taking together, these results imply that phosphorylation of CPAP on serine 1109 is required for 14-3-3 binding. Furthermore, we observed that the interaction between CPAP and 14-3-3 was significantly reduced in mitotic cells, suggesting that 14-3-3 binding to CPAP is regulated during cell cycle progression. In summary, our results show a direct interaction between CPAP and 14-3-3, and this interaction appears to be phosphorylation and cell cycle depe...Continue Reading

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Citations

Jun 9, 2009·Nature Cell Biology·Chieh-Ju C TangTang K Tang
Jun 5, 2007·Nature Genetics·Anneke I den HollanderRonald Roepman
Nov 26, 2009·Progress in Neurobiology·Angela M KaindlPierre Gressens
Sep 30, 2020·Cells·Rustem E Uzbekov, Tomer Avidor-Reiss

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