CpG-containing ODN has a limited role in the protection against Toxoplasma gondii

Parasite Immunology
R SaavedraP Hérion

Abstract

Bacterial DNA containing immunostimulatory motifs (CpG) induces the development of a T(H1) immune response. Since protection against Toxoplasma gondii is correlated with this type of response, the aim of this work was to determine if a synthetic oligodeoxynucleotide (ODN) containing CpG sequences could be useful as adjuvant for the induction of a long-lasting protective immune response against T. gondii. BALB/c mice immunized with a total soluble antigen of T. gondii (TSA2) mixed with ODN-containing CpG sequences developed a typical TH1 response, as determined by antibody isotypes and interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) production by spleen cells. However, they did not resist a challenge with the virulent RH strain of the parasite. Absence of protection paralleled with lower levels of IFN-gamma, when compared with mice vaccinated with the live tachyzoites of the attenuated ts.4 strain of the parasite, which resisted this challenge. Intraperitoneal injection of ODN alone to mice induced a high degree of resistance to a lethal challenge inoculated by the same route. Nevertheless, this nonspecific protection was transient. Thus, the use of ODN containing CpG motifs as adjuvant is of limited value for the inductio...Continue Reading

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Citations

Jul 9, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Marcos FloresMiriam Rodriguez-Sosa
Jul 4, 2007·Proceedings of the American Thoracic Society·Arthur M Krieg
Jan 30, 2010·Journal of Biomedicine & Biotechnology·Yuriko SanchezMiriam Rodríguez-Sosa
Aug 30, 2005·International Journal for Parasitology·Luis I TerrazasMiriam Rodríguez-Sosa
Feb 19, 2019·Clinical and Experimental Vaccine Research·Masoud ForoutanOgholniaz Jorjani

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