CpG immunostimulatory oligodeoxynucleotide 1826 enhances antitumor effect of interleukin 12 gene-modified tumor vaccine in a melanoma model in mice

Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
Tomasz SwitajW Lasek

Abstract

The effectiveness of interleukin (IL)-12-secreting tumor vaccines in the treatment of mouse tumors could be enhanced by concurrent application of cytokines and costimulatory molecules. We investigated the therapeutic potential of IL-12 gene-transduced melanoma vaccine in combination with CpG immunostimulatory oligodeoxynucleotide (ODN) 1826, an adjuvant known to favor development of Th1-biased immune response, in a B78-H1 (B78) melanoma model in mice. Mice injected with B78 melanoma cells were treated with irradiated IL-12 gene-transduced B78 cells [B78/IL-12(X)] and/or ODN 1826. Mechanisms responsible for the antitumor effects of the treatment were investigated using fluorescence-activated cell sorter analysis, a standard (51)Cr releasing assay, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and ELISA. Single injection of B78/IL-12(X) cells had no effect on tumor growth, whereas seven consecutive daily injections of ODN 1826 markedly inhibited tumor progression with occasional curative effects. When used in combination, B78/IL-12(X) cells and ODN 1826 caused additional tumor growth reduction and eradication of tumors in 62% of treated mice. The combined treatment activated local inflammatory response again...Continue Reading

References

Apr 19, 1995·Journal of the National Cancer Institute·E E VoestJ Folkman
Jun 6, 2000·International Immunology·E BanG Riveau
Jul 21, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·G B LipfordH Wagner
Sep 28, 2000·Current Opinion in Immunology·N Mach, G Dranoff
Feb 13, 2001·Cell·A Aderem, D A Hume
Feb 13, 2001·Vaccine·P Moingeon
Apr 5, 2001·Trends in Immunology·I MeleroJ Prieto
May 24, 2001·Immunity·H Wagner
Aug 18, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·K H BaekY C Sung
Oct 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·Z K BallasG J Weiner
Feb 28, 2002·Annual Review of Immunology·Arthur M Krieg
Mar 20, 2002·Cytokine & Growth Factor Reviews·Mario P Colombo, Giorgio Trinchieri
Jun 11, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Britta V SternMagdalena Tary-Lehmann
Aug 23, 2002·Cancer Immunology, Immunotherapy : CII·Stephen WardStephen Todryk
Sep 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Klaus HeckelsmillerGunther Hartmann
Nov 28, 2002·International Journal of Cancer. Journal International Du Cancer·Stephan R SchefferTim F Greten
Mar 5, 2003·Annual Review of Immunology·Drew Pardoll
Mar 22, 2003·Cancer Immunology, Immunotherapy : CII·Johanna E A PortieljeWim H J Kruit
Dec 23, 2003·Immunology Letters·Marek JakóbisiakJakub Gołab

❮ Previous
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Citations

Feb 15, 2011·Cancer Immunology, Immunotherapy : CII·Hidekazu Shirota, Dennis M Klinman
Dec 1, 2005·Photodiagnosis and Photodynamic Therapy·Dominika NowisJakub Gołąb
Feb 12, 2014·Cancer Immunology, Immunotherapy : CII·Witold LasekMarek Jakobisiak
Oct 6, 2004·Expert Opinion on Biological Therapy·Piotr J WysockiA Mackiewicz
May 5, 2007·Expert Opinion on Biological Therapy·P J WysockiA Mackiewicz
Jan 27, 2009·Advanced Drug Delivery Reviews·David M Lubaroff, Dev Karan
Aug 9, 2008·International Immunopharmacology·Hyeon Cheol ChoSoo-Ki Kim
May 28, 2013·Seminars in Immunology·Ramon ArensCornelis J M Melief
Dec 3, 2013·Bioorganic & Medicinal Chemistry·Tien-Lam TranWon-Keun Oh
Oct 11, 2016·PLoS Genetics·Maximilian StahlThomas Prebet
Feb 13, 2018·Journal of Leukocyte Biology·Andra BaneteSameh Basta
Oct 11, 2017·ACS Biomaterials Science & Engineering·Nicholas M MolinoSzu-Wen Wang

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