CpG promoter hypo-methylation and up-regulation of microRNA-190b in hormone receptor-positive breast cancer

Oncotarget
Elisabet FrickStefan Sigurdsson

Abstract

Estrogen receptor-positive breast cancer is subdivided into subtypes LuminalA and LuminalB, based on different expression patterns. MicroRNA-190b has been reported to be up-regulated in estrogen receptor-positive breast cancers. In this study we aimed to investigate the role of CpG promoter methylation in regulating miR-190b expression and its impact on clinical presentation and prognosis. DNA methylation analysis for the promotor of microRNA-190b was performed by pyrosequencing 549 primary breast tumors, of which 62 were carriers of the BRCA2 999del5 founder mutation, 71 proximal normal breast samples and 16 breast derived cell lines. MicroRNA-190b expression was analysed in 67 primary breast tumors, 14 paired normal breast samples and 16 breast derived cell lines. Tissue microarrays (TMAs) were available for ER (n = 436), PR (n = 436), HER-2 (N = 258) and Ki67 (n = 248). MiR-190b had reduced promoter methylation in estrogen receptor-positive breast cancers (P = 1.02e-12, Median values: ER+ 24.3, ER- 38.26) and miR-190b's expression was up-regulated in a correlative manner (P = 1.83e-06, Spearman's rho -0.62). Through breast cancer specific survival analysis, we demonstrated that LuminalA patients exhibiting miR-190b hypo-met...Continue Reading

References

Aug 30, 2000·Nature·C M PerouD Botstein
Sep 13, 2001·Proceedings of the National Academy of Sciences of the United States of America·T SørlieA L Børresen-Dale
Sep 23, 2008·International Journal of Cancer. Journal International Du Cancer·Tae-Min KimYeun-Jun Chung
Jul 11, 2009·Breast Cancer Research : BCR·Olafur Andri StefanssonJorunn Erla Eyfjord
Jan 23, 2010·Nature Reviews. Molecular Cell Biology·Richard J JacksonTatyana V Pestova
May 25, 2010·Trends in Molecular Medicine·Gianpiero Di Leva, Carlo M Croce
Dec 16, 2010·The Oncologist·Martine J Piccart-Gebhart
Jul 15, 2011·Journal of the National Cancer Institute·William F AndersonPhilip S Rosenberg
Oct 1, 2011·Breast Cancer Research : BCR·Olafur A StefanssonJorunn E Eyfjord
Sep 15, 2012·Acta Oncologica·Lara Gudrun SigurdardottirLaufey Tryggvadottir
Sep 20, 2012·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Reina HaqueMichael F Press
Apr 4, 2013·Science Signaling·Jianjiong GaoNikolaus Schultz
Aug 21, 2013·PLoS Computational Biology·Michael LawrenceVincent J Carey
Oct 1, 2013·The Breast : Official Journal of the European Society of Mastology·Philippe BertheauHugues de Thé
Oct 12, 2013·CA: a Cancer Journal for Clinicians·Carol DeSantisAhmedin Jemal
Jan 23, 2014·Nature Communications·Krishna L KanchiLi Ding
Jun 11, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Tao TaoUNKNOWN Chinese Glioma Cooperative Group (CGCG)
Jul 23, 2014·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Felipe AdesMartine Piccart
Aug 20, 2014·Current Biology : CB·Brian D AdamsFrank J Slack
Oct 7, 2015·Trends in Cell Biology·Hiro-Oki Iwakawa, Yukihide Tomari
Feb 28, 2016·Nucleic Acids Research·Nicole LudwigAndreas Keller
Mar 24, 2016·Methods in Molecular Biology·Florian Hahne, Robert Ivanek
May 3, 2016·Nature·Serena Nik-ZainalMichael R Stratton
Jun 29, 2016·Journal of Experimental & Clinical Cancer Research : CR·Laura PaladiniLibero Santarpia
Jan 1, 2015·The Journal of Pathology. Clinical Research·Margret AradottirSigridur K Bodvarsdottir
Aug 19, 2016·British Journal of Cancer·Jon G JonassonLaufey Tryggvadóttir
Sep 23, 2016·The New England Journal of Medicine·Robert L GrossmanLouis M Staudt
Aug 24, 2017·Nature Communications·Kara N MaxwellKatherine L Nathanson
Nov 21, 2017·Nucleic Acids Research·Daniel R ZerbinoPaul Flicek
Jan 14, 2018·Molecular Cancer Research : MCR·Dongwook KimYounghee Lee
Jul 3, 2018·Frontiers in Oncology·Christy W S TongKenneth K W To

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Methods Mentioned

BETA
Assay
PCR

Software Mentioned

R
Genomic Ranges
Bioconductor
PyroMark Q24
Gviz
biomaRt

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