CpsB is a modulator of capsule-associated tyrosine kinase activity in Streptococcus pneumoniae

The Journal of Biological Chemistry
M H Bender, J Yother

Abstract

Tyrosine phosphorylation is associated with polysaccharide synthesis in a number of Gram-positive and Gram-negative bacteria. In Streptococcus pneumoniae, CpsB, CpsC, and CpsD affect tyrosine phosphorylation and are critical for the production of a mature capsule in vitro. To characterize the interactions between these proteins and the phosphorylation event they modulate, cps2B, cps2C, and cps2D from the capsule type 2 S. pneumoniae D39 were cloned and expressed both individually and in combination in Escherichia coli. Cps2D purified from E. coli was not phosphorylated unless it was co-expressed with its cognate transmembrane domain, Cps2C. Purified phosphorylated Cps2D had tyrosine kinase activity and could phosphorylate both dephosphorylated Cps2D and an exogenous substrate (poly-Glu-Tyr) in the absence of ATP. Cps2B exhibited phosphatase activity against both purified phosphorylated Cps2D and p-nitrophenyl phosphate. An additional role for Cps2B as an inhibitor of Cps2D phosphorylation was demonstrated in both co-expression experiments in E. coli and in vitro experiments where it blocked the transphosphorylation of Cps2D even in the presence of the phosphatase inhibitor sodium orthovanadate. cps2C and cps2D deletion mutants ...Continue Reading

References

Apr 1, 1992·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·D M Musher
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·C E RubensD L Kasper
Mar 1, 1981·Reviews of Infectious Diseases·J A Winkelstein
Oct 1, 1995·Journal of Clinical Microbiology·J Henrichsen
Apr 9, 1998·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·M J CaimanoJ Yother
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·J E GonzálezG C Walker
Apr 10, 1999·Journal of Bacteriology·F IannelliG Pozzi
Apr 14, 1999·Molecular Microbiology·C Whitfield, I S Roberts
Feb 8, 2000·The Journal of Biological Chemistry·R T CarteeJ Yother
May 12, 2001·Infection and Immunity·A D Magee, J Yother
Jul 1, 1949·The Journal of Experimental Medicine·W B WOOD, M R SMITH
Jun 30, 1931·The Journal of Experimental Medicine·O T Avery, R Dubos
Jan 1, 1928·The Journal of Hygiene·F Griffith

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Citations

Aug 24, 2002·Journal of Bacteriology·Didier SoulatAlain J Cozzone
May 4, 2005·Journal of Bacteriology·Ivan MijakovicNunzio Bottini
Jul 5, 2005·Journal of Bacteriology·Lucia MusumeciNunzio Bottini
Oct 8, 2014·Microbiology·Alistair J StandishRenato Morona
Sep 2, 2016·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·G ErricoR Camilli
Jun 9, 2004·Free Radical Biology & Medicine·Sumio YanoNoriko Yano
Oct 14, 2017·FEMS Microbiology Reviews·Ditte Høyer EngholmRikke Schmidt Kjærgaard
Jul 26, 2002·The Journal of Biological Chemistry·Patricia DoubletAlain J Cozzone
Mar 6, 2012·International Journal of Oral Science·Sarah E Whitmore, Richard J Lamont
Jun 12, 2013·Canadian Journal of Microbiology·Angela D CefaloDennis L Welker
Dec 26, 2001·The Journal of Biological Chemistry·Christophe GrangeasseAlain J Cozzone
Feb 21, 2014·The Journal of Biological Chemistry·Joseph D ChaoYossef Av-Gay
Feb 11, 2015·The Journal of Biological Chemistry·Chiara TonioloRobert Janulczyk
Jul 4, 2017·Frontiers in Pharmacology·Shuai WangYanhua Li
Jun 27, 2009·Chemical Communications : Chem Comm·Maximilian Pitscheider, Stephan A Sieber
May 1, 2020·Frontiers in Microbiology·Monika Pióro, Dagmara Jakimowicz
Dec 19, 2020·FEMS Microbiology Reviews·Tong SuLok-To Sham
Apr 13, 2019·Microbiology Spectrum·James C Paton, Claudia Trappetti
Sep 27, 2005·Biochimica Et Biophysica Acta·Josef DeutscherSandrine Poncet

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