CPX, a selective A1-adenosine-receptor antagonist, regulates intracellular pH in cystic fibrosis cells

The American Journal of Physiology
V CasavolaHarvey B Pollard

Abstract

The selective A1-adenosine-receptor antagonist, 8-cyclopentyl-1,3-dipropylxanthine (CPX), has been reported to activate Cl- efflux from cystic fibrosis cells, such as pancreatic CFPAC-1 and lung IB3 cells bearing the cystic fibrosis transmembrane regulator(delta F508) mutation, but has little effect on the same process in cells repaired by transfection with wild-type cystic fibrosis transmembrane regulator (O. Eidelman, C. Guay-Broder, P. J. M. van Galen, K. A. Jacobson, C. Fox, R. J. Turner, Z. I. Cabantchik, and H. B. Pollard. Proc. Natl. Acad. Sci. USA 89: 5562-5566, 1992). We report here that CPX downregulates Na+/H+ exchange activity in CFPAC-1 cells but has a much smaller effect on cells repaired with the wild-type gene. CPX also mildly decreases resting intracellular pH. In CFPAC-1 cells, this downregulation is dependent on the presence of adenosine, since pretreatment of the cells with adenosine deaminase blocks the CPX effect. We also show that, by contrast, CPX action on these cells does not lead to alterations in intracellular free Ca2+ concentration. We conclude that CPX affects pH regulation in CFPAC-1 cells, probably by antagonizing the tonic action of endogenous adenosine.

References

Jan 1, 1976·The Journal of General Physiology·W F Boron, P De Weer
Aug 1, 1992·British Journal of Pharmacology·E R LazarowskiR C Boucher
Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·N ArispeH B Pollard
Apr 15, 1992·Biochemical and Biophysical Research Communications·R W Van DykeJ M Wilson
Apr 24, 1992·Science·N A BradburyK L Kirk
Nov 1, 1992·American Journal of Respiratory Cell and Molecular Biology·E PuchelleW Dalemans
Jan 1, 1991·Advances in Experimental Medicine and Biology·M A McPhersonR L Dormer
Feb 1, 1991·Journal of Cellular Physiology·D H Sohn, H D Kim
Jan 1, 1991·Advances in Experimental Medicine and Biology·M R KnowlesR C Boucher
Jul 1, 1990·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·P M Quinton
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·L L ClarkeR C Boucher
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·U KerstingK R Spring
Sep 1, 1993·American Journal of Respiratory Cell and Molecular Biology·C CarnoyP Roussel

❮ Previous
Next ❯

Citations

Oct 29, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A ZsemberyJ Graf
Dec 16, 2000·Expert Opinion on Investigational Drugs·D J Wallace
Nov 18, 2000·American Journal of Physiology. Cell Physiology·L BulteauF Becq
Jan 22, 2002·Pediatric Pulmonology·Nael A McCartyRichard C Ahrens
Apr 13, 2015·Reproduction, Fertility, and Development·J M ConnollyA C Hynes
Dec 16, 2000·Expert Opinion on Investigational Drugs·G M Roomans
Jun 27, 2002·American Journal of Pharmacogenomics : Genomics-related Research in Drug Development and Clinical Practice·O EidelmanH B Pollard
Jul 28, 2012·Age·Jeffrey A StuartEllen L Robb
Feb 19, 2008·Thyroid : Official Journal of the American Thyroid Association·Mary-Ellen Harper, Erin L Seifert
Jun 1, 1999·Journal of Genetic Counseling·T Brown, E L Schwind
Oct 14, 2005·Biological Reviews of the Cambridge Philosophical Society·Douglas S Glazier
Oct 26, 2005·Biochemical Society Transactions·M D Brand
Mar 27, 2002·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·W M WebeW Van Driessche
Sep 1, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Isabel Virella-LowellTerence R Flotte
Oct 6, 1998·Clinics in Chest Medicine·P L Zeitlin
Jun 17, 2005·Nutrition & Metabolism·Christopher B Scott
Nov 15, 2011·Gastroenterology·Marcelo A CatalánJames E Melvin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.