Cr supplementation decreases tyrosine phosphorylation of the CreaT in skeletal muscle during sepsis

American Journal of Physiology. Endocrinology and Metabolism
W WangD O Jacobs

Abstract

Myocellular creatine (Cr) uptake is predominantly governed by a sodium-dependent Cr transporter (CreaT) and plays a pivotal role in skeletal muscle energy metabolism. The CreaT belongs to a neurotransmitter transporter family that can be functionally regulated by protein tyrosine kinase-induced tyrosine phosphorylation. The association between myocellular Cr and c-Src-related tyrosine phosphorylation of the CreaT and the influence of oral Cr supplementation on this association were investigated during sepsis. Animals were randomized to receive standard rat chow or standard rat chow with oral Cr supplementation for 4 days followed by cecal ligation and puncture (CLP) or sham operation. Fast-twitch gastrocnemius muscles were harvested 24 h after operation. Myocellular free Cr levels were 70% higher after CLP. Western blotting of the immunoprecipitated CreaT with an anti-phosphotyrosine or anti-phospho-c-Src (Y-416) antibody revealed that tyrosine phosphorylation of the CreaT and tyrosine-phosphorylated c-Src (Tyr(416)) expression in the CreaT-c-Src complex were significantly increased after CLP compared with sham operation. These changes were observed in homogenates and plasma membrane fractions of gastrocnemius muscles. Although...Continue Reading

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Citations

May 27, 2008·Journal of the International Society of Sports Nutrition·Ryan D SchochMike Greenwood
Mar 13, 2012·The Journal of Membrane Biology·Manzar ShojaiefardFlorian Lang
Jul 23, 2005·Biochemical and Biophysical Research Communications·Manzar ShojaiefardFlorian Lang
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Mar 4, 2003·Journal of Applied Physiology·Jeffrey J BraultRonald L Terjung
May 30, 2013·American Journal of Physiology. Endocrinology and Metabolism·Sevasti ZervouCraig A Lygate

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