Abstract
Normal human B lymphocytes activate the alternative pathway of complement via complement receptor type 2 (CR2, CD21), that binds hydrolysed C3 (iC3) and thereby promotes the formation of a membrane-bound C3 convertase. We have investigated whether this might lead to the generation of a C5 convertase and consequent formation of membrane attack complexes (MAC). Deposition of C3 fragments and MAC was assessed on human peripheral B lymphocytes in the presence of 30% autologous serum containing 4.4 mM MgCl2/20 mM EGTA, which abrogates the classical pathway of complement without affecting the alternative pathway. Blockade of the CR2 ligand-binding site with the monoclonal antibody FE8 resulted in 56 +/- 13% and 71 +/- 9% inhibition of the C3-fragment and MAC deposition, respectively, whereas the monoclonal antibody HB135, directed against an irrelevant CR2 epitope, had no effect. Blockade of the CR1 binding site with the monoclonal antibody 3D9 also resulted in a minor reduction in MAC deposition, while FE8 and 3D9, in combination, markedly reduced deposition of both C3 fragments (91 +/- 5%) and C9 (95 +/- 3%). The kinetics of C3-fragment and MAC deposition, as well as the dependence of both processes on CR2, indicate that MAC format...Continue Reading
References
May 1, 1991·The Journal of Experimental Medicine·D A TuvesonD T Fearon
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·O F RamosJ Gergely
Jan 1, 1985·The Journal of Membrane Biology·T Wiedmer, P J Sims
Aug 1, 1985·Scandinavian Journal of Immunology·T E MollnesJ Tschopp
Jun 1, 1983·European Journal of Immunology·R G MedicusM A Arnaout
May 1, 1981·The Journal of Experimental Medicine·K Iida, V Nussenzweig
Sep 1, 1995·The American Journal of Physiology·C M Padgett, A R Whorton
Apr 1, 1995·Cell Calcium·I LaffafianM B Hallett
May 1, 1993·American Journal of Hematology·S NagakuraK Takatsuki
Mar 1, 1994·Cell Calcium·J C PapadimitriouB F Trump
Jan 1, 1993·Immunologic Research·A Nicholson-Weller, J A Halperin
Oct 7, 1997·Scandinavian Journal of Immunology·H V MarquartR G Leslie
Jun 9, 1998·Immunology·E H OlesenR G Leslie
Jan 14, 1999·International Immunology·K KerekesA Erdei
Jun 11, 1999·Journal of the American Society of Nephrology : JASN·T SatoM R Daha
Aug 14, 1999·Immunology·R G Leslie
Nov 7, 1999·The American Journal of Pathology·A V CybulskyA J McTavish
May 23, 2000·European Journal of Immunology·S Kraus, Z Fishelson
Citations
Aug 20, 2003·The Journal of Experimental Medicine·Joerg Rossbacher, Mark J Shlomchik
Sep 25, 2009·Immunology·Morten LøbnerClaus H Nielsen
Dec 18, 2003·Immunology·Robert Graham Quinton Leslie, Claus Henrik Nielsen
Apr 24, 2010·Transplant International : Official Journal of the European Society for Organ Transplantation·Takafumi MachimotoJuan Scornik
Jan 29, 2011·Molecular Immunology·Liudmila KulikV Michael Holers
Sep 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Cody A KochJeffrey L Platt
Mar 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Amanda C JacobsonJohn H Weis
Aug 8, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sherry D FlemingV Michael Holers