CRA(Crosolic Acid) isolated from Actinidia valvata Dunn.Radix induces apoptosis of human gastric cancer cell line BGC823 in vitro via down-regulation of the NF-κB pathway

Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
Qi-Lai ChengYi-Jian Cheng

Abstract

A natural ursolic compound, 2α,3β-dihydroxy-urs-12-en-28-oic acid (corosolic acid, CRA) was isolated from the root of Actinidia valvata Dunn. (A. valvata Radix). Since a large number of triterpenoid compound has marked anticancer effects toward various types of cancer cell lines in vitro, this study was carried out to investigate the anticancer effect of CRA in human gastric cancer cell line BGC823 cells and the underlying apoptotic mechanism of CRA was examined in BGC823 cell lines. The results showed that CRA significantly suppressed the viability of BGC823 cells in a concentration- and time-dependent manner. CRA also significantly increased the sub G1 population by cell cycle analysis in a concentration dependent manner. Exposure to CRA decreased p65, bcl-2, Fas, smac mRNA and protein expression, and increased IκBα, bax, survivin mRNA and protein expression. Results of immunofluorescence staining and EMSA further indicated CRA induced apoptosis by inhibiting nuclear translocation of nuclear factor NF-κB subunit p65. Consistently overall, our findings suggest that CRA induces apoptosis via inhibition of NF-κB (p65) expression level and activation of IκBα in BGC cells as a potent anticancer candidate for gastric cancer treatment.

Citations

Nov 21, 2019·Bioscience Reports·Zhao-Peng WangYuan Yuan
Feb 28, 2019·Documenta Ophthalmologica. Advances in Ophthalmology·Cibele Rodrigues ToledoArmando Silva-Cunha
Jun 6, 2018·Cancer Biomarkers : Section a of Disease Markers·Chen-Guang LiuJian-Sheng Guo
Aug 31, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Xu-Ping QianYong-Qing Wang

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