Cranial neural crest deletion of VEGFa causes cleft palate with aberrant vascular and bone development

Cell and Tissue Research
Cynthia R HillSteven Goudy

Abstract

Cleft palate is among the most common craniofacial congenital anomalies. Up to 30% of patients with cleft palate also have associated cardiac and vascular defects. VEGFa, a critical growth factor involved in multiple developmental processes including angiogenesis and ossification, is also required for palate development. Conditional deletion of VEGFa in cranial neural crest (CNC) cells using Wnt1-Cre (VEGFaCKO) resulted in cleft palate in mice. The phenotype included reduced proliferation of cells within the palatal shelves, abnormal palatal shelf elongation and elevation, and the inability to undergo fusion. Vascularization of the VEGFaCKO palatal shelves was greatly reduced, suggesting a non-cell autonomous role of VEGFa signaling from the CNC-derived cells to the endothelium during vessel formation. Defective vascular development was coupled with deficient intramembranous ossification of maxillary and palatal mesenchyme. In vitro assessment of CNC-derived palatal mesenchymal cells from VEGFaCKO mice demonstrated normal ossification after BMP2 stimulation, suggesting that inadequate expression of Bmp2 in VEGFaCKO mice was, in part, responsible for reduced ossification. Taken together, these data demonstrate that VEGFa produce...Continue Reading

References

Jul 1, 1990·The Cleft Palate Journal·R K WyseK D Blake
Aug 25, 1999·Microvascular Research·C L GrosskreutzP A D'Amore
Sep 2, 1999·Human Molecular Genetics·B C Schutte, J C Murray
Oct 25, 2002·Journal of Cellular Biochemistry·Jörg FiedlerRolf E Brenner
Jan 23, 2003·Nature Medicine·Ingeborg StalmansPeter Carmeliet
Jun 5, 2003·Nature Medicine·Napoleone FerraraJennifer LeCouter
Jun 18, 2003·The Journal of Cell Biology·Holger GerhardtChrister Betsholtz
Jun 17, 2004·The Journal of Clinical Investigation·Ritva RiceDavid P C Rice
Jan 12, 2005·Current Topics in Developmental Biology·Elazar Zelzer, Bjorn R Olsen
Jul 12, 2005·Biochemical and Biophysical Research Communications·Jörg FiedlerRolf E Brenner
May 9, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Yang Chai, Robert E Maxson
Sep 1, 2006·Developmental Biology·Amel Gritli-Linde
Jul 12, 2008·Mechanisms of Development·Toshiyuki YoshidaSachiko Iseki
Apr 2, 2009·Organogenesis·Jody J Haigh
Mar 11, 2010·Mechanisms of Development·Steven GoudyChristopher Brown
Dec 28, 2010·Developmental Biology·Jin-A BaekRulang Jiang
Jan 10, 2012·Genesis : the Journal of Genetics and Development·Thomas DoetschmanMohamad Azhar
Feb 2, 2012·International Journal of Molecular Medicine·Jun MizutaniTakanobu Otsuka
Feb 3, 2012·International Journal of Biological Sciences·Guiqian ChenYi-Ping Li
Feb 14, 2012·The Journal of Clinical Investigation·Jun-ichi IwataYang Chai
Oct 17, 2012·The Laryngoscope·James A OwusuAndrew R Scott

❮ Previous
Next ❯

Citations

Feb 24, 2016·Matrix Biology : Journal of the International Society for Matrix Biology·Xuchen DuanAgnes D Berendsen
Oct 18, 2016·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Kai Hu, Bjorn R Olsen
Jul 1, 2017·The American Journal of Chinese Medicine·Yan-Mei ShuiJian-Li Gao
Nov 23, 2017·Frontiers in Bioengineering and Biotechnology·Andrea GrossoNunzia Di Maggio
Feb 19, 2020·The Cleft Palate-craniofacial Journal : Official Publication of the American Cleft Palate-Craniofacial Association·Evija Balode, Mara Pilmane
Nov 30, 2019·Scientific Reports·Robin M H RumneyRichard O C Oreffo
Aug 28, 2020·Frontiers in Cell and Developmental Biology·Eva SvandovaHerve Lesot
Sep 3, 2020·Tissue Engineering. Part B, Reviews·Jeremie D OliverRena N D'Souza
Jan 11, 2020·Frontiers in Cell and Developmental Biology·Katiúcia Batista Silva PaivaAriadne Letra
Mar 5, 2019·Laryngoscope Investigative Otolaryngology·Anita S Deshpande, Steven L Goudy
Oct 30, 2020·Frontiers in Cell and Developmental Biology·Miranda R SunRobert J Lipinski
Aug 20, 2020·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Frank C Ko, D Rick Sumner
Jun 5, 2021·Mutation Research. Reviews in Mutation Research·Ghenwa NasreddineMichella Ghassibe-Sabbagh

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Birth Defects

Birth defects encompass structural and functional alterations that occur during embryonic or fetal development and are present since birth. The cause may be genetic, environmental or unknown and can result in physical and/or mental impairment. Here is the latest research on birth defects.

Cardiac Conduction System

The cardiac conduction system is a specialized tract of myocardial cells responsible for maintaining normal cardiac rhythm. Discover the latest research on the cardiac conduction system here.