Crayfish mechanoreceptor neuron prevents photoinduced apoptosis of satellite glial cells

Brain Research Bulletin
Mikhail Kolosov, Anatoly Uzdensky

Abstract

Interactions between neurons and glia play a key role in the development, functioning and survival of the nervous system. However, the influence of neurons on glial cells has received less attention than the role of glia in supporting neural functions. We here investigated the role of isolated crayfish stretch receptor neuron in the death of satellite glial cells under photodynamic impact. After staining with aluminum phthalocyanine photosens, the neuronal cell body was locally irradiated with a focused beam of He-Ne (633 nm, 200 W/cm2) or semiconductor laser (650 nm, 50 W/cm2). This rapidly abolished neuronal activity. The whole preparation was then subjected to total laser irradiation with lower intensity (633 nm, 0.3 W/cm2), which induced death of glial cells. Double staining of the preparation with propidium iodide and Hoechst 33342 in the following 6-7h allowed the visualization of necrotic, apoptotic and alive cells. Previous neuron inactivation with the focused laser beam was found to increase photodynamically-induced apoptosis but not necrosis of satellite glial cells enwrapping the axon. Therefore, the intact neuronal cell body protected satellite glial cells against photoinduced apoptosis. Altogether the data indicate...Continue Reading

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Citations

Jul 9, 2009·Cell and Tissue Research·Grigory M Fedorenko, Anatoly B Uzdensky
Apr 22, 2009·Journal of Molecular Neuroscience : MN·A V Lobanov, A B Uzdensky
Aug 1, 2012·Journal of Molecular Neuroscience : MN·Anatoly UzdenskyAndrej Lobanov
Oct 31, 2006·Photomedicine and Laser Surgery
Nov 28, 2007·Zhurnal evoliutsionnoĭ biokhimii i fiziologii·A V Lobanov, A B Uzdenskiĭ
Jan 10, 2013·Nitric Oxide : Biology and Chemistry·Vera KovalevaAnatoly Uzdensky
Dec 7, 2007·Journal of Neuroscience Research·G M Fedorenko, A B Uzdensky
Oct 27, 2016·Journal of Biomedical Optics·Vera D Kovaleva, Anatoly B Uzdensky
Aug 23, 2008·Neuroscience and Behavioral Physiology·A B UzdenskiiA V Lobanov
Sep 13, 2020·Journal of Molecular Neuroscience : MN·Valentina DzreyanAnatoly Uzdensky

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis