Crayfish tissue metabolomes effectively distinguish impacts of wastewater and agriculture in aquatic ecosystems

The Science of the Total Environment
Natalie M IzralAdam G Yates

Abstract

Environmental metabolomics has been proposed as a tool for biomonitoring because organisms regulate production or consumption of metabolites in response to environmental conditions. We evaluated the efficacy of the metabolome of three tissues (hepatopancreas, gill, and tail muscle) from the northern crayfish (Faxonius virilis) to detect and differentiate between impacts of human activities (i.e., reference, municipal wastewater, and agriculture). We conducted a reciprocal transfer study exposing crayfish for 1 or 2 weeks in three streams with different amounts and types of human activities in southern Manitoba, Canada. Tissue samples were analyzed using nuclear magnetic resonance spectroscopy to generate a metabolic profile. Findings indicated the gill tissue metabolome best detected and differentiated between human activities. In particular, the gill metabolome was able to rapidly integrate abrupt changes in environmental conditions associated with municipal wastewater activity. In contrast, the tail metabolome best differentiated between crayfish collected at the reference site from those collected at the two impacted sites. Metabolites extracted from hepatopancreas tissue showed limited and inconsistent detection of among si...Continue Reading

References

Dec 15, 1972·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·G Schwoch
May 16, 1998·BMJ : British Medical Journal·T V Perneger
Mar 19, 2002·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Tamaki Fujimori, Hiroki Abe
Nov 19, 2003·Environmental Science & Technology·Mark R ViantRonald S Tieerdema
Jul 10, 2004·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·G A AhearnA Mandal
Oct 30, 2007·Analytical Biochemistry·Huifeng WuMark R Viant
Dec 17, 2008·Molecular BioSystems·Mark R Viant
May 5, 2012·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Christopher P BonvillainChristopher C Green
Jul 24, 2012·Ecotoxicology and Environmental Safety·Salvatore FasuloAngela Mauceri
Nov 12, 2013·Marine Pollution Bulletin·Tiziana CappelloSalvatore Fasulo
Apr 5, 2014·Ophthalmic & Physiological Optics : the Journal of the British College of Ophthalmic Opticians (Optometrists)·Richard A Armstrong
Apr 24, 2014·Environmental Science and Pollution Research International·Amanda Gago-TinocoJosé-Luis Gómez-Ariza
Aug 22, 2016·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Stuart R Green, Kenneth B Storey
Oct 27, 2018·Environmental Science and Pollution Research International·Natalie M IzralAdam G Yates
Nov 23, 2019·Current Protocols in Bioinformatics·Jasmine ChongJianguo Xia

❮ Previous
Next ❯

Citations

Jul 3, 2021·Frontiers in Molecular Biosciences·Sarah M PomfretAdam G Yates

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

JAMA : the Journal of the American Medical Association
F Lang
Nature Reviews. Drug Discovery
Sarah Crunkhorn
© 2021 Meta ULC. All rights reserved