Cre-dependent expression of multiple transgenes in isolated neurons of the adult forebrain.

PloS One
Sridhara ChakravarthyChristiaan N Levelt

Abstract

Transgenic mice with mosaic, Golgi-staining-like expression of enhanced green fluorescent protein (EGFP) have been very useful in studying the dynamics of neuronal structure and function. In order to further investigate the molecular events regulating structural plasticity, it would be useful to express multiple proteins in the same sparse neurons, allowing co-expression of functional proteins or co-labeling of subcellular compartments with other fluorescent proteins. However, it has been difficult to obtain reproducible expression in the same subset of neurons for direct comparison of neurons expressing different functional proteins. Here we describe a Cre-transgenic line that allows reproducible expression of transgenic proteins of choice in a small number of neurons of the adult cortex, hippocampus, striatum, olfactory bulb, subiculum, hypothalamus, superior colliculus and amygdala. We show that using these Cre-transgenic mice, multiple Cre-dependent transgenes can be expressed together in the same isolated neurons. We also describe a Cre-dependent transgenic line expressing a membrane associated EGFP (EGFP-F). Crossed with the Cre-transgenic line, EGFP-F expression starts in the adolescent forebrain, is present in dendrites...Continue Reading

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Jan 19, 2006·Proceedings of the National Academy of Sciences of the United States of America·Sridhara ChakravarthyChristiaan N Levelt

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Citations

Oct 1, 2010·Reviews in the Neurosciences·Martijn Dahlhaus, Christiaan N Levelt
Jul 26, 2011·Journal of Chemical Neuroanatomy·José L Lanciego, Floris G Wouterlood

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Methods Mentioned

BETA
transgenic
confocal microscopy
imaging techniques

Software Mentioned

Olympus
Fluoview

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