Creatine affords protection against glutamate-induced nitrosative and oxidative stress

Neurochemistry International
Mauricio P CunhaAna Lúcia S Rodrigues

Abstract

Creatine has been reported to exert beneficial effects in several neurodegenerative diseases in which glutamatergic excitotoxicity and oxidative stress play an etiological role. The purpose of this study was to investigate the protective effects of creatine, as compared to the N-Methyl-d-Aspartate (NMDA) receptor antagonist dizocilpine (MK-801), against glutamate or hydrogen peroxide (H2O2)-induced injury in human neuroblastoma SH-SY5Y cells. Exposure of cells to glutamate (60-80 mM) or H2O2 (200-300 μM) for 24 h decreased cellular viability and increased dichlorofluorescein (DCF) fluorescence (indicative of increased reactive oxygen species, ROS) and nitric oxide (NO) production (assessed by mono-nitrogen oxides, NOx, levels). Creatine (1-10 mM) or MK-801 (0.1-10 μM) reduced glutamate- and H2O2-induced toxicity. The protective effect of creatine against glutamate-induced toxicity involves its antioxidant effect, since creatine, similar to MK-801, prevented the increase on DCF fluorescence induced by glutamate or H2O2. Furthermore, creatine or MK-801 blocked glutamate- and H2O2-induced increases in NOx levels. In another set of experiments, the repeated, but not acute, administration of creatine (300 mg/kg, po) in mice prevente...Continue Reading

References

Nov 1, 1992·Archives of Biochemistry and Biophysics·H IschiropoulosJ S Beckman
Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J S StamlerJ Loscalzo
Jan 1, 1992·Neuron·D S Bredt, S H Snyder
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M E Murphy, H Sies
Sep 1, 1990·Trends in Pharmacological Sciences·B Meldrum, J Garthwaite
Jan 1, 1984·Critical Reviews in Toxicology·J DiGuiseppi, I Fridovich
Jan 1, 1983·Annual Review of Pharmacology and Toxicology·I Fridovich
Oct 27, 1995·Journal of Medicinal Chemistry·J F KerwinP L Feldman
Nov 13, 1995·Biochemical and Biophysical Research Communications·C BehlF Holsboer
Feb 1, 1996·Molecular Neurobiology·F BlandiniJ T Greenamyre
Jan 1, 1997·Free Radical Biology & Medicine·C M Pereira, C R Oliveira
Feb 25, 1999·International Journal of Geriatric Psychiatry·R CacabelosB Winblad
May 1, 1999·Experimental Neurology·R T MatthewsM F Beal
Oct 19, 1999·Brain Research. Brain Research Reviews·F TorreillesJ Torreilles
Apr 13, 2000·Brain Research. Brain Research Reviews·A Contestabile
Oct 24, 2000·Biochimica Et Biophysica Acta·T WeiA Mori
Nov 18, 2000·Annals of Neurology·P G SullivanS W Scheff
Feb 13, 2001·Trends in Neurosciences·D E BarañanoS H Snyder
Nov 22, 2001·Experimental Neurology·S VielhaberW S Kunz
Jan 10, 2002·Biochemical and Biophysical Research Communications·John M LawlerScott Demaree
Mar 6, 2002·British Journal of Pharmacology·Juan V Esplugues
Apr 4, 2003·The New England Journal of Medicine·Barry ReisbergUNKNOWN Memantine Study Group
Jul 6, 2004·Neurochemistry International·Matthew R HyndPeter R Dodd
Dec 27, 2005·The Veterinary Journal·Simon R Platt
Feb 14, 2006·Pharmacology, Biochemistry, and Behavior·Luiz Fernando Freire RoyesCarlos Fernando Mello
Jan 24, 2007·Physiological Reviews·Pál PacherLucas Liaudet
Sep 21, 2007·Nature Reviews. Neuroscience·Vittorio CalabreseAnna Maria Giuffrida Stella
Nov 21, 2007·Biochimica Et Biophysica Acta·Chiara GuidiVilberto Stocchi
May 27, 2008·Brain Research Bulletin·Robert H AndresHans Rudolf Widmer

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Citations

Feb 5, 2019·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jolyn FernandesDean P Jones
Aug 28, 2019·Biomolecules·Brent M KiousPerry F Renshaw
Apr 25, 2019·Neurotoxicity Research·Marcos Roberto de OliveiraClaudia Marlise Balbinotti Andrade
Jun 14, 2020·Molecular Neurobiology·Rodrigo ZanandreaCarla Denise Bonan
Nov 26, 2020·Cellular and Molecular Neurobiology·Zhenzhen XuRenshi Xu
Jan 16, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Gabriel GonzalezMiroslav Strnad

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