PMID: 8944630Nov 1, 1996Paper

Creatine depletion elicits structural, biochemical, and physiological adaptations in rat costal diaphragm

The American Journal of Physiology
S LevineH L Sweeney

Abstract

To elucidate adaptations elicited by creatine (Cr) depletion in the costal diaphragm (Dia), 16 12-wk-old male Fisher 344 rats had 2% beta-guanidinopropionic acid (beta-GPA), a competitive inhibitor of Cr transport into muscle, added to their food; a control group (Con) of 16 rats ate normal rat chow. After 18 wk, beta-GPA and Con Dia did not differ histochemically with respect to fiber-type distribution; however, the cross-sectional area of type II(b + x) fibers was 33% less in beta-GPA than Con Dia. Biochemically, the proportion of myosin heavy chain IIb in beta-GPA Dia was decreased 42% from Con Dia, whereas the proportions of myosin heavy chains I and IIa were increased. Physiologically, both peak twitch tension and tetanic tension in beta-GPA Dia were decreased 40% from Con. To assess fatigability, we used the protocol of Kelsen and Nochomovitz (J. Appl. Physiol. 53; 440-447, 1982) for 2-6 min duration; the percentage of initial force exhibited by beta-GPA Dia was approximately twice that of Con Dia. We conclude that these structural, biochemical, and physiological adaptations elicited by Cr depletion can all be explained by selective atrophy of IIb muscle fibers in the Dia.

References

Apr 1, 1975·The American Journal of Physiology·C D FitchJ O Holloszy
Jun 1, 1989·Journal of Muscle Research and Cell Motility·S SchiaffinoT Lømo
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Nov 1, 1993·Journal of Applied Physiology·R J Talmadge, R R Roy

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Citations

Dec 18, 1997·The New England Journal of Medicine·S LevineB Tikunov
Jul 14, 2000·Physiological Reviews·M Wyss, R Kaddurah-Daouk
Mar 19, 2008·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Darren G Candow, Philip D Chilibeck
Oct 23, 2002·Clinical and Experimental Pharmacology & Physiology·Dean M Robinson, Denis S Loiselle

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