Creatine supplementation prevents hyperhomocysteinemia, oxidative stress and cancer-induced cachexia progression in Walker-256 tumor-bearing rats

Amino Acids
Rafael DeminiceFlávia Alessandra Guarnier

Abstract

The purpose of this study was to investigate (1) the impact of tumor growth on homocysteine (Hcy) metabolism, liver oxidative stress and cancer cachexia and, (2) the potential benefits of creatine supplementation in Walker-256 tumor-bearing rats. Three experiments were conducted. First, rats were killed on days 5 (D5), 10 (D10) and 14 (D14) after tumor implantation. In experiment 2, rats were randomly assigned to three groups designated as control (C), tumor-bearing (T) and tumor-bearing supplemented with creatine (TCr). A life span experiment was conducted as the third experiment. Creatine was supplied in drinking water for 21 days (8 g/L) in all cases. Tumor implantation consisted of a suspension of Walker-256 cells (8.0 × 10(7) cells in 0.5 mL of PBS). The progressive increase (P < 0.05) in tumor mass coincided with a progressively lower body weight and higher hepatic oxidative stress; plasma Hcy concentration was 80 % higher (P < 0.05) by 10 days of tumor implantation. Impaired Hcy metabolism was evidenced by decreased hepatic betaine-homocysteine methyltransferase (Bhmt), glycine N-methyltransferase (Gnmt) and cystathionine beta synthase (CBS) gene expression. In contrast, creatine supplementation promoted a 28 % reduction...Continue Reading

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Citations

Jul 17, 2016·Amino Acids·Theo Wallimann, Roger Harris
Sep 16, 2016·Frontiers in Pharmacology·Priyank A ShenoyMaree T Smith
Jun 16, 2017·Journal of the International Society of Sports Nutrition·Richard B KreiderHector L Lopez
Nov 15, 2019·Journal of Applied Physiology·Megan E Rosa-CaldwellNicholas P Greene
Jun 20, 2019·Oxidative Medicine and Cellular Longevity·Jin WangWei Chen
Jan 9, 2021·Frontiers in Nutrition·Wouter R P H van de WorpRamon C J Langen
Oct 27, 2016·Current Opinion in Supportive and Palliative Care·Riccardo BallaròFabio Penna
Feb 13, 2021·Nutrients·Richard B Kreider, Jeffery R Stout

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