Creating Hierarchical Topographies on Fibrous Platforms Using Femtosecond Laser Ablation for Directing Myoblasts Behavior

ACS Applied Materials & Interfaces
Indong JunHojeong Jeon

Abstract

Developing an artificial extracellular matrix that closely mimics the native tissue microenvironment is important for use as both a cell culture platform for controlling cell fate and an in vitro model system for investigating the role of the cellular microenvironment. Electrospinning, one of the methods for fabricating structures that mimic the native ECM, is a promising technique for creating fibrous platforms. It is well-known that align or randomly distributed electrospun fibers provide cellular contact guidance in a single pattern. However, native tissues have hierarchical structures, i.e., topographies on the micro- and nanoscales, rather than a single structure. Thus, we fabricated randomly distributed nanofibrous (720 ± 80 nm in diameter) platforms via a conventional electrospinning process, and then we generated microscale grooves using a femtosecond laser ablation process to develop engineered fibrous platforms with patterned hierarchical topographies. The engineered fibrous platforms can regulate cellular adhesive morphology, proliferation, and distinct distribution of focal adhesion proteins. Furthermore, confluent myoblasts cultured on the engineered fibrous platforms revealed that the direction of myotube assembly...Continue Reading

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Citations

Jun 9, 2016·ACS Applied Materials & Interfaces·Hongmiao TianYucheng Ding
Mar 7, 2018·International Journal of Molecular Sciences·Indong JunHojeong Jeon
Nov 28, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Young Min ShinHeung Jae Chun
Nov 9, 2018·Frontiers in Bioengineering and Biotechnology·Anna DenchaiElisa Mele
Jan 14, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Young Min ShinHeungsoo Shin
Jan 5, 2019·ACS Applied Materials & Interfaces·Romina RegaSimonetta Grilli
Oct 8, 2018·ACS Biomaterials Science & Engineering·Ayesha ArefinJennifer F Harris

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