Creating hippocampal PPI connectivity maps with SPM12 batch files v1

Douglas D. Burman

Abstract

Protocol for task-specific, effective connectivity analysis of hippocampus using SPM12 batch files and PPI analysis. This protocol requires input from an SPM analysis on smoothed, normalized, slice-time corrected data (files with swa prefix) using 4mm isovoxels, with the first contrast specifying effects of interest (an F-contrast, excluding any movement covariates but specifying every experimental and control condition). The batch files in this protocol generate connectivity maps from each voxel in both the left and right hippocampus, then generates averaged connectivity maps from specified regions of the hippocampus, eliminating the necessity to identify seed regions a priori from methods (such as activation) not directly related to connectivity analysis. This procedure can be repeated for as many as 30 subjects. The final step creates a random effects group analysis for each of 9 bilateral structural seeds, as described elsewhere (e.g., Burman, 2021 "Topography of hippocampal connectivity with sensorimotor cortex revealed by optimizing smoothing kernel and voxel size", doi: https://doi.org/10.1101/2020.05.14.096339); however, connectivity maps created by the protocol allow an investigator to run group analysis on any region ...Continue Reading

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