Creating tissues from textiles: scalable nonwoven manufacturing techniques for fabrication of tissue engineering scaffolds

Biomedical Materials
S A TuinE G Loboa

Abstract

Electrospun nonwovens have been used extensively for tissue engineering applications due to their inherent similarities with respect to fibre size and morphology to that of native extracellular matrix (ECM). However, fabrication of large scaffold constructs is time consuming, may require harsh organic solvents, and often results in mechanical properties inferior to the tissue being treated. In order to translate nonwoven based tissue engineering scaffold strategies to clinical use, a high throughput, repeatable, scalable, and economic manufacturing process is needed. We suggest that nonwoven industry standard high throughput manufacturing techniques (meltblowing, spunbond, and carding) can meet this need. In this study, meltblown, spunbond and carded poly(lactic acid) (PLA) nonwovens were evaluated as tissue engineering scaffolds using human adipose derived stem cells (hASC) and compared to electrospun nonwovens. Scaffolds were seeded with hASC and viability, proliferation, and differentiation were evaluated over the course of 3 weeks. We found that nonwovens manufactured via these industry standard, commercially relevant manufacturing techniques were capable of supporting hASC attachment, proliferation, and both adipogenic and...Continue Reading

References

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Citations

Aug 18, 2017·Tissue Engineering. Part a·Thomas L JenkinsDianne Little
Sep 20, 2018·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Shaobo CaiElizabeth G Loboa
Jul 9, 2020·Soft Matter·Ting Yang Nilsson, Markus Andersson Trojer
May 21, 2019·Journal of Biomaterials Science. Polymer Edition·Thalles Canton TrevisolAna Paula Serafini Immich
Jul 3, 2019·Npj Regenerative Medicine·Thomas Lee Jenkins, Dianne Little
Jun 20, 2020·Tissue Engineering. Part C, Methods·Rohan A ShirwaikerBehnam Pourdeyhimi
Apr 16, 2021·Chemical Reviews·Alina KirillovaMatthew L Becker

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