Creation of a three-dimensional spherical fluorescence spot for super-resolution microscopy using a two-color annular hybrid wave plate

Optics Letters
Yoshinori IketakiNandor Bokor

Abstract

We fabricate a two-color annular hybrid wave plate (TAHWP) for fluorescence depletion super-resolution microscopy by combining quartz plates. Inserting the TAHWP into a commercial laser-scanning microscope together with a quarter-wave plate creates a three-dimensional spherical fluorescence spot smaller than the diffraction limit. Since a simple experimental setup easily provides a spot with a volume of ∼100  nm<sup>3</sup>, the TAHWP can improve the performance of various microspectroscopic devices.

References

Jul 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·T A KlarS W Hell
Nov 5, 2003·Nature Biotechnology·Stefan W Hell
Apr 22, 2006·The Journal of Organic Chemistry·Akimitsu OkamotoYoshimasa Fujiwara
Sep 3, 2009·Optics Express·Dominik WildangerLars Kastrup
Sep 24, 2009·Journal of Microscopy·D WildangerS W Hell
Oct 6, 2012·Optics Express·Travis J GouldMartin J Booth
Mar 29, 2014·Applied Spectroscopy·Nandor Bokor, Yoshinori Iketaki

❮ Previous
Next ❯

Citations

Apr 26, 2017·Optics Express·Xiaoyu WengXiaocong Yuan
Nov 10, 2018·Scientific Reports·J C TungT Omatsu
May 5, 2018·Applied Optics·Zhongsheng ManShenggui Fu
Jan 1, 2021·Optics Express·A Srinivasa RaoTakashige Omatsu
Dec 1, 2019·Nanophotonics·Jialei TangKyu Young Han

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.