Creation of an antibody-based subcellular protein atlas

Proteomics
Emma Lundberg, Mathias Uhlén

Abstract

An important part for understanding the complex machinery of living cells is to know the spatial distribution of proteins all the way from organ to organelle levels. An equally important part of proteomics is to map the subcellular distribution of all human proteins. Here, we discuss methodologies for systematic subcellular profiling with emphasis on the antibody-based approach performed as a part of the Human Protein Atlas project. The considerations made when creating the subcellular protein atlas and critical parameters of this approach are discussed.

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Citations

Apr 11, 2013·Chemical Reviews·Chad P SatoriEdgar A Arriaga
Jul 6, 2011·Journal of Proteome Research·Linn FagerbergAnna Asplund
Jan 2, 2013·Journal of Proteome Research·Linn FagerbergMathias Uhlén
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Sep 16, 2014·Journal of Proteome Research·Adil MardinogluJens Nielsen
Aug 31, 2017·Journal of Proteome Research·Gilbert S OmennEric W Deutsch

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The aim of the Human Cell Atlas (HCA) is to build reference maps of all human cells in order to enhance our understanding of health and disease. The Seed Networks for the HCA project aims to bring together collaborators with different areas of expertise in order to facilitate the development of the HCA. Find the latest research from members of the HCA Seed Networks here.