Creation of BAC genomic resources for cocoa ( Theobroma cacao L.) for physical mapping of RGA containing BAC clones

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
D ClémentP Piffanelli

Abstract

We have constructed and validated the first cocoa ( Theobroma cacao L.) BAC library, with the aim of developing molecular resources to study the structure and evolution of the genome of this perennial crop. This library contains 36,864 clones with an average insert size of 120 kb, representing approximately ten haploid genome equivalents. It was constructed from the genotype Scavina-6 (Sca-6), a Forastero clone highly resistant to cocoa pathogens and a parent of existing mapping populations. Validation of the BAC library was carried out with a set of 13 genetically-anchored single copy and one duplicated markers. An average of nine BAC clones per probe was identified, giving an initial experimental estimation of the genome coverage represented in the library. Screening of the library with a set of resistance gene analogues (RGAs), previously mapped in cocoa and co-localizing with QTL for resistance to Phytophthora traits, confirmed at the physical level the tight clustering of RGAs in the cocoa genome and provided the first insights into the relationships between genetic and physical distances in the cocoa genome. This library represents an available BAC resource for structural genomic studies or map-based cloning of genes corr...Continue Reading

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Citations

Sep 1, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·T LeroyP Piffanelli
Oct 1, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·D A LalliA G Abbott
Jan 26, 2007·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Maria MarcanoClaire Lanaud
Nov 21, 2008·Molecular Plant Pathology·Lyndel W MeinhardtGonçalo A G Pereira
Jun 11, 2014·Critical Reviews in Food Science and Nutrition·Neela BadrieMarek Sikora
Jun 15, 2007·Annals of Botany·Abelmon S GesteiraJúlio C M Cascardo

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