Creation of Bifunctional Indicating Complex Based on Nanodiamonds and Extracellular Oxidases of Luminous Fungus Neonothopanus nambi

Doklady. Biochemistry and Biophysics
O A MogilnayaV S Bondar

Abstract

A bifunctional indicating complex was created by immobilization of extracellular oxidases (glucose oxidase and peroxidases) of luminous fungus Neonothopanus nambi onto modified nanodiamonds (MNDs) synthesized by detonation. It was found that the enzymes firmly adsorb onto MND particles and exhibit their catalytic activity. Model in vitro experiments showed that the created MND-enzymes complex is suitable for repeated use for analyte (glucose and phenol) testing and retains its activity after storage at 4°C in deionized water for 1 month. The data obtained offer the prospects for developing a new class of reusable multifunctional indicating and diagnostic test systems on the basis of MNDs and higher fungal enzymes for medical and ecological analytics.

References

Mar 12, 2008·Applied Microbiology and Biotechnology·Chun Ming WongXiao Dong Chen
May 25, 2010·Applied Microbiology and Biotechnology·Martin HofrichterTaina Lundell
Dec 14, 2011·Analytical Chemistry·Francis P ZamboriniRadhika Dasari
Nov 16, 2013·Journal of Colloid and Interface Science·Paula CiaurrizKimberly Hamad-Schifferli
Feb 20, 2014·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Hao YangSongqin Liu

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Citations

Apr 29, 2020·Doklady. Biochemistry and Biophysics·N O RonzhinV S Bondar

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