Creation of deletion and insertion clonal complex 8 Staphylococcus aureus mutants using a common cloning vector

Journal of Microbiological Methods
Ping ChenKai P Leung

Abstract

Deletion and insertion clonal complex 8 Staphylococcus aureus mutants were created without using intermediate host S. aureus RN4220 or temperature-sensitive shuttle vectors. These mutants were created using a common cloning vector by passing the constructs through a modification host and recovering the electroporated cells in a large volume of medium.

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