Creek to Table: Tracking fecal indicator bacteria, bacterial pathogens, and total bacterial communities from irrigation water to kale and radish crops

The Science of the Total Environment
Sarah M AllardAmy R Sapkota

Abstract

The impact of microbially contaminated irrigation water on risks to produce safety and public health is a complex issue that is not well understood. This study tracked fecal indicators, pathogenic bacteria, and total bacterial communities from a creek water irrigation source to irrigated produce to assess the impact of irrigation events on soil and produce-associated microbiota. Kale and radishes were drip-irrigated using Mid-Atlantic creek water in October 2017. Plant and soil samples were collected immediately before and after irrigation, and for 3 consecutive days thereafter. All samples (n = 134), including irrigation water, were tested for generic Escherichia coli and total coliforms (TC) using standard membrane filtration or direct plating, and for Salmonella enterica and Listeria monocytogenes by selective enrichment. DNA extracted from all samples was PCR-amplified for the V3-V4 region of the 16S rRNA gene for bacterial community profiling. In soil, TC levels were significantly higher immediately and 3 days post-irrigation compared to pre-irrigation (p < 0.01). E. coli levels in soil increased after irrigation, but the difference was not significant (p = 0.31), and die-off was not observed. No E. coli were detected on k...Continue Reading

References

Jun 2, 1998·The Journal of Infectious Diseases·M L AckersL Slutsker
Nov 6, 2004·Applied and Environmental Microbiology·Eva Møller NielsenDorte L Baggesen
Jan 7, 2005·Journal of Food Protection·Marina Steele, Joseph Odumeru
Feb 9, 2008·Emerging Infectious Diseases·Michele T JayRobert E Mandrell
Jan 7, 2009·Applied and Environmental Microbiology·Bradd J HaleyErin K Lipp
Jul 8, 2009·Foodborne Pathogens and Disease·Irene B HanningSteven C Ricke
Apr 13, 2010·Nature Methods·J Gregory CaporasoRob Knight
Jun 28, 2011·Bioinformatics·Robert C EdgarRob Knight
Feb 16, 2012·BMC Bioinformatics·Andre P MasellaJosh D Neufeld
Oct 1, 2013·Nature Methods·Joseph N PaulsonMihai Pop
Aug 12, 2014·Applied and Environmental Microbiology·Baoguang LiChristopher A Elkins
Aug 31, 2014·Applied and Environmental Microbiology·Rachel McEganMichelle D Danyluk
Dec 18, 2014·Genome Biology·Michael I LoveSimon Anders
Dec 30, 2014·International Journal of Food Microbiology·Sivaranjani PagadalaRobert L Buchanan
Dec 26, 2015·Environmental Monitoring and Assessment·Ryan A BlausteinYakov A Pachepsky
Jan 6, 2016·Applied and Environmental Microbiology·Neiunna L Reed-JonesShirley A Micallef
Apr 17, 2016·Applied and Environmental Microbiology·Mary Theresa CallahanRobert L Buchanan
May 22, 2016·Applied and Environmental Microbiology·I DoutereloJ Boxall
Feb 22, 2017·Journal of Food Protection·Shirley A MicallefSivaranjani Pagadala
May 10, 2018·Frontiers in Microbiology·Jessica ChopykAmy R Sapkota

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