CrELISA: a fast and robust enzyme-linked immunosorbent assay bypassing the need for purification of recombinant protein

Journal of Immunological Methods
Ozlem TüreciUgur Sahin

Abstract

A multitude of antigens has been recently identified by screening of cDNA expression libraries derived from human tumors with autologous sera. Using a phage autoantibody assay and small panels of sera derived from cancer patients or controls it has been shown that some of these antigens display cancer-associated autoantibody responses. The diagnostic and prognostic significance of these potentially cancer-related autoantibodies remains unclear until large-scale assays are developed and serological data are available for hundreds of cancer patients and controls. The major bottleneck for the development of large-scale assays are the cloning, expression and the purification of each of the respective antigens. Due to these limitations and despite the potential clinical relevance large-scale autoantibody tests are established for only a few of these tumor antigens. Here we describe an enzyme-linked immunosorbent assay, Crude lysate ELISA (CrELISA), suitable for antigens identified by expression screening based on crude lysates of antigen-expressing bacteria. This assay permits sensitive and specific autoantibody seroscreening without the need of laborious and time-consuming cloning, expression and purification of recombinant protein...Continue Reading

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Citations

Sep 30, 2005·Cancer Immunology, Immunotherapy : CII·F L ZhouS H Liu
May 31, 2008·BMC Immunology·Vivian GoodellMary L Disis
Sep 10, 2015·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Andreas KuemmelRoland Buhl
May 26, 2005·Journal of Immunological Methods·Vivian Goodell, Mary L Disis
Jan 18, 2007·Expert Opinion on Therapeutic Targets·Hailing LuMary L Disis

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