CRF receptor type 1 and 2 expression and anatomical distribution in the rat colon

Journal of Neurochemistry
Ekaterini ChatzakiD E Grigoriadis

Abstract

Corticotropin-releasing factor (CRF) receptor agonists administered peripherally increase colonic propulsive motility and fecal output in experimental animals. In addition, endogenous CRF-related peptides are found in the lower gastrointestinal (GI) tissues, suggesting a local expression of CRF receptors. In the present study, we report the expression of both CRF receptor type 1 (CRF(1)) and 2 (CRF(2)) in the rat colon at the mRNA and protein levels. For the purpose of receptor protein mapping, a specific antiserum against the C-terminus of CRF(2) (2064a-CRF(2)) was generated and characterized. This antiserum in conjunction with a selective anti-CRF(1) antiserum (4467a-CRF(1)) was used in immunofluorescent staining to demonstrate the anatomical distribution of receptor protein expression. Using RT-PCR for the CRF(1) and CRF(2) genes, both receptor gene transcripts were found in RNA isolated from crude colonic samples. CRF(1) was found in the goblet and stem cells of the colonic crypts and in scattered cells of the surface epithelium and the lamina propria of the proximal colonic mucosa. In addition, double staining against neuron-specific antigens revealed CRF(1) expression in the myenteric and submucosal nervous plexus. CRF(2)...Continue Reading

Citations

Oct 3, 2009·Reviews in Endocrine & Metabolic Disorders·Willemijntje A Hoogerwerf
May 2, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Muriel LaraucheYvette Taché
Feb 19, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jen ChangAditi Bhargava
Nov 9, 2006·Neuroendocrinology·Ioannis CharalampopoulosAchille Gravanis
Jan 4, 2007·The Journal of Clinical Investigation·Yvette Taché, Bruno Bonaz
Jan 24, 2007·Pediatric Research·Jayaraman LakshmananMichael G Ross
Oct 1, 2010·Experimental Biology and Medicine·Andreas Stengel, Yvette Taché
Mar 30, 2013·World Journal of Gastroenterology : WJG·Ekaterini ChatzakiDimitri E Grigoriadis
May 19, 2005·Proceedings of the National Academy of Sciences of the United States of America·Nigel W Bunnett
Feb 10, 2010·Cellular and Molecular Life Sciences : CMLS·Athina KapraraEkaterini Chatzaki
Nov 28, 2015·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·S LiuA Bhargava
Oct 16, 2012·Gastroenterology·Bruno L Bonaz, Charles N Bernstein
Apr 22, 2009·Drug Discovery Today·Konstantinos A PaschosEkaterini Chatzaki
Jan 1, 2008·General and Comparative Endocrinology·Hugo Mendieta-ZerónCarlos Diéguez
Dec 23, 2006·Biochemical Pharmacology·Bengt von MentzerVicente Martínez
Apr 7, 2004·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Y Taché, M H Perdue
Nov 21, 2008·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·J LakshmananM G Ross
Mar 1, 2012·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·A C JohnsonB Greenwood-Van Meerveld
Dec 5, 2012·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·T NozuT Okumura
Feb 13, 2010·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·A V KeitaA-C Ericson
Oct 8, 2009·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·D o'malleyJ F Cryan
Jan 18, 2006·General and Comparative Endocrinology·Graham C Boorse, Robert J Denver
May 13, 2015·Journal of Gastroenterology·Tsukasa Nozu, Toshikatsu Okumura
Dec 30, 2014·Journal of Neurogastroenterology and Motility·Bruno K Rodiño-JaneiroJavier Santos

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.