Crimean-Congo Hemorrhagic Fever Virus Nucleocapsid Protein Augments mRNA Translation

Journal of Virology
Subbiah JeevaM A Mir

Abstract

Crimean-Congo hemorrhagic fever virus (CCHFV) is a tick-borne Nairovirus of theBunyaviridaefamily, causing severe illness with high mortality rates in humans. Here, we demonstrate that CCHFV nucleocapsid protein (CCHFV-NP) augments mRNA translation. CCHFV-NP binds to the viral mRNA 5' untranslated region (UTR) with high affinity. It facilitates the translation of reporter mRNA bothin vivoandin vitrowith the assistance of the viral mRNA 5' UTR. CCHFV-NP equally favors the translation of both capped and uncapped mRNAs, demonstrating the independence of this translation strategy on the 5' cap. Unlike the canonical host translation machinery, inhibition of eIF4F complex, an amalgam of three initiation factors, eIF4A, eIF4G, and eIF4E, by the chemical inhibitor 4E1RCat did not impact the CCHFV-NP-mediated translation mechanism. However, the proteolytic degradation of eIF4G alone by the human rhinovirus 2A protease abrogated this translation strategy. Our results demonstrate that eIF4F complex formation is not required but eIF4G plays a critical role in this translation mechanism. Our results suggest that CCHFV has adopted a unique translation mechanism to facilitate the translation of viral mRNAs in the host cell cytoplasm where cel...Continue Reading

References

Jan 1, 1987·The American Journal of Tropical Medicine and Hygiene·R SwanepoelD E Gill
Oct 1, 1993·The Journal of General Virology·H Jin, R M Elliott
Jun 6, 2000·Microbiology and Molecular Biology Reviews : MMBR·M GaleM G Katze
Apr 30, 2003·Journal of Virology·Ramon FlickFredrik Elgh
Nov 20, 2004·Antiviral Research·Chris A Whitehouse
Oct 31, 2008·The EMBO Journal·Mohammad A Mir, Antonito T Panganiban
Dec 3, 2008·Proceedings of the National Academy of Sciences of the United States of America·M A MirA T Panganiban
Oct 20, 2009·Nature Structural & Molecular Biology·Wataru KamitaniShinji Makino
Jul 14, 2010·The Journal of Biological Chemistry·Nogi ParkKurt E Gustin
Sep 17, 2010·Journal of Virology·Absarul Haque, Mohammad A Mir
Nov 19, 2010·Nature·Xiaoxuan QiChangjiang Dong
Dec 31, 2010·Proceedings of the National Academy of Sciences of the United States of America·Regina CencicJerry Pelletier
Jan 26, 2011·Proceedings of the National Academy of Sciences of the United States of America·Kathryn M HastieErica Ollmann Saphire
Nov 16, 2011·Proceedings of the National Academy of Sciences of the United States of America·Kathryn M HastieErica Ollmann Saphire
Mar 17, 2012·Proceedings of the National Academy of Sciences of the United States of America·Yu GuoZihe Rao
Jul 13, 2012·Journal of Virology·Erdong Cheng, Mohammad A Mir
Sep 30, 2016·Journal of Virology·Erin WalkerReena Ghildyal
Oct 14, 2016·The Journal of Biological Chemistry·Nilshad N SalimMohammad A Mir

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Citations

Apr 6, 2019·Archives of Virology·Yusuke MatsumotoMachiko Nishio
Nov 22, 2017·Virus Genes·Monika Reuter, Detlev H Krüger
Dec 14, 2018·Journal of Virology·Subbiah JeevaMohammad Mir
Feb 13, 2019·Reviews in Medical Virology·Sebastiaan Ter HorstJoana Rocha-Pereira
Jan 9, 2021·ELife·David W HawmanHeinz Feldmann
Nov 23, 2021·PloS One·Austin RoysterMohammad Ayoub Mir

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