CRISPR-based reagents to study the influence of the epigenome on gene expression

Clinical and Experimental Immunology
P LavenderP McErlean

Abstract

The use of epigenome editing is set to expand our knowledge of how epigenetic landscapes facilitate gene expression capacity within a given cell. As epigenetic landscape profiling in health and disease becomes more commonplace, so does the requirement to assess the functional impact that particular regulatory domains and DNA methylation profiles have upon gene expression capacity. That functional assessment is particularly pertinent when analysing epigenomes in disease states where the reversible nature of histone and DNA modification might yield plausible therapeutic targets. In this review we discuss first the nature of the epigenetic landscape, secondly the types of factors that deposit and erase the various modifications, consider how modifications transduce their signals, and lastly address current tools for experimental epigenome editing with particular emphasis on the immune system.

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Citations

Dec 24, 2018·Expert Review of Clinical Immunology·Niklas BruseMatthijs Kox
Apr 24, 2020·Molecular Diagnosis & Therapy·Jeffrey D Rubin, Michael A Barry
Dec 29, 2020·Frontiers in Genetics·Peter McErleanPaul Lavender
Jul 20, 2021·American Journal of Respiratory and Critical Care Medicine·Peter McErleanAdam J Byrne

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Methods Mentioned

BETA
flow cytometry
ChIPseq
histone acetylation
acetylation
immunoprecipitation

Software Mentioned

ENCODE
Roadmap

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