CRISPR-Cas systems in multicellular cyanobacteria

RNA Biology
Shengwei HouWolfgang R Hess

Abstract

Novel CRISPR-Cas systems possess substantial potential for genome editing and manipulation of gene expression. The types and numbers of CRISPR-Cas systems vary substantially between different organisms. Some filamentous cyanobacteria harbor > 40 different putative CRISPR repeat-spacer cassettes, while the number of cas gene instances is much lower. Here we addressed the types and diversity of CRISPR-Cas systems and of CRISPR-like repeat-spacer arrays in 171 publicly available genomes of multicellular cyanobacteria. The number of 1328 repeat-spacer arrays exceeded the total of 391 encoded Cas1 proteins suggesting a tendency for fragmentation or the involvement of alternative adaptation factors. The model cyanobacterium Anabaena sp. PCC 7120 contains only three cas1 genes but hosts three Class 1, possibly one Class 2 and five orphan repeat-spacer arrays, all of which exhibit crRNA-typical expression patterns suggesting active transcription, maturation and incorporation into CRISPR complexes. The CRISPR-Cas system within the element interrupting the Anabaena sp. PCC 7120 fdxN gene, as well as analogous arrangements in other strains, occupy the genetic elements that become excised during the differentiation-related programmed site-...Continue Reading

Citations

Jan 31, 1995·Proceedings of the National Academy of Sciences of the United States of America·Claudio D CarrascoJames W Golden
Jan 1, 1994·Genes & Development·Claudio D CarrascoJames W Golden
Jan 1, 1996·Annual Review of Genetics·C P Wolk
Mar 1, 1997·Molecular Microbiology·K S RamaswamyJames W Golden
Aug 20, 2005·Journal of Bacteriology·Claudio D CarrascoJames W Golden
Feb 20, 2008·Archives of Microbiology·B J HensonS R Barnum
Jul 5, 2008·Genome Research·Jeff ElhaiJosé Luis Costa
Feb 28, 2009·Microbiology·Francisco J M MojicaC Almendros
Jun 10, 2009·Bioinformatics·Salvador Capella-GutiérrezToni Gabaldón
May 11, 2010·Cold Spring Harbor Perspectives in Biology·Krithika KumarJames W Golden
Sep 25, 2010·Research in Microbiology·Shiraz A Shah, Roger A Garrett
Feb 22, 2011·Bioinformatics·Diego DarribaDavid Posada
Sep 29, 2011·Trends in Microbiology·Roger A GarrettShiraz A Shah
Oct 13, 2011·Molecular Systems Biology·Fabian SieversDesmond G Higgins
Dec 3, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jan MitschkeAlicia M Muro-Pastor
Jan 19, 2013·Molecular Biology and Evolution·Kazutaka Katoh, Daron M Standley
Feb 14, 2013·RNA Biology·Shiraz A ShahRoger A Garrett
Aug 14, 2013·Proceedings of the National Academy of Sciences of the United States of America·Ido YosefUdi Qimron
Jan 18, 2014·Molecular Microbiology·Susanne ErdmannRoger A Garrett
Mar 20, 2014·Bioinformatics·Torsten Seemann
Nov 29, 2014·Science·Jennifer A Doudna, Emmanuelle Charpentier
Feb 25, 2015·Nature·James K NuñezJennifer A Doudna
Oct 16, 2015·Nucleic Acids Research·Yingjun LiQunxin She
Nov 25, 2015·Molecular Cell·Sergey ShmakovEugene V Koonin
Dec 18, 2015·Nucleic Acids Research·Robert D FinnAlex Bateman
Apr 21, 2016·Nature·De DongZhiwei Huang
May 24, 2016·Molecular Cell·James K NuñezJennifer A Doudna
Sep 3, 2016·Bioinformatics·Omer S AlkhnbashiRolf Backofen
Sep 8, 2016·Nucleic Acids Research·Viktoria ReimannWolfgang R Hess
Nov 16, 2016·Molecular BioSystems·L Dorosh, M Stepanova
Dec 22, 2016·Scientific Reports·Justin Ungerer, Himadri B Pakrasi
Jan 24, 2017·Nature Reviews. Microbiology·Sergey ShmakovEugene V Koonin

Related Concepts

Conserved Synteny
Study
Gene Expression Regulation, Bacterial
MRNA Maturation
Untranslated Regions
Abnormal Fragmented Structure
Genome
Genes
CRISPR-Cas Systems
Complex (molecular entity)

Related Feeds

CRISPR (general)

Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). CRISPR-Cas system enables the editing of genes to create or correct mutations. Discover the latest research on CRISPR here.

CRISPR Ribonucleases Deactivation

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on mechanisms that underlie deactivation of CRISPR ribonucleases. Here is the latest research.

CRISPR for Genome Editing

Genome editing technologies enable the editing of genes to create or correct mutations. Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). Here is the latest research on the use of CRISPR-Cas system in gene editing.