Jul 2, 2016

CRISPR-Cas9-Guided Genome Engineering in C. elegans

Current Protocols in Molecular Biology
Hyun-Min Kim, Monica P Colaiácovo

Abstract

The CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated) system is successfully being used for efficient and targeted genome editing in various organisms, including the nematode C. elegans. Recent studies have developed various CRISPR-Cas9 approaches to enhance genome engineering via two major DNA double-strand break repair pathways: non-homologous end joining and homologous recombination. Here we describe a protocol for Cas9-mediated C. elegans genome editing together with single guide RNA (sgRNA) and repair template cloning, as well as injection methods required for delivering Cas9, sgRNAs, and repair template DNA into the C. elegans germline. © 2016 by John Wiley & Sons, Inc.

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Citations

Mentioned in this Paper

Calcinus elegans
Cyartonema elegans
Study
Coleonyx elegans
Non-Homologous DNA End-Joining
Biochemical Pathway
Cestrum elegans
Clarkia unguiculata
Clathrulina elegans
Cardioglossa elegans

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