Apr 23, 2020

Fruit bromelain derived peptides destabilize growth of amyloidal fibrils

BioRxiv : the Preprint Server for Biology
S. DasDebasish Bhattacharyya

Abstract

{beta}-Amyloid deposition as fibrillar plaques in brain is the primary cause of Alzheimer's disease. We report potency of cysteine protease 'fruit bromelain' from pineapple in destabilising A{beta} fibrils. Bromelain peptide pool (Mw<500 Da) obtained mimicking human alimentary tract digestion inhibited fibrillation from monomeric and oligomeric states of A{beta} and irreversibly dissociated preformed fibrils into small oligomers of varied sizes. Time kinetics was followed by Thioflavin-T assay and microscopic imaging. Synthetic bromelain peptides corresponding to A{beta} sticky region found using ClustalW analysis revealed specificity of peptides in destabilisation of amyloidal structures. Spectra of different molecular states of A{beta} obtained from application of 8-anilino-1-naphthalenesulfonic acid, circular dichroism and Fourier-Transformed Infrared spectroscopy collectively indicated interaction dependent structural change. Probable mechanism for fibril dissociation was thus predicted. Peptides relieved A{beta} cytotoxicity on pheochromcytoma cells and dissociated plaques in AD-type rats prepared by bilateral intracerebroventricular administration of A{beta} in rat brain cortex. Pineapple being a phytoceutical, its effici...Continue Reading

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Mentioned in this Paper

C9orf72 protein, human
Pluripotent Stem Cells
Pathogenic Organism
Nerve Degeneration
Gene Deletion Abnormality
Intermediate Periventricular Nucleus
Gene Deletion
Promoter
Amyotrophic Lateral Sclerosis
Excision

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