Dec 10, 2017

CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing

Nature Communications
Miguel A Moreno-MateosAntonio J Giraldez

Abstract

Cpf1 is a novel class of CRISPR-Cas DNA endonucleases, with a wide range of activity across different eukaryotic systems. Yet, the underlying determinants of this variability are poorly understood. Here, we demonstrate that LbCpf1, but not AsCpf1, ribonucleoprotein complexes allow efficient mutagenesis in zebrafish and Xenopus. We show that temperature modulates Cpf1 activity by controlling its ability to access genomic DNA. This effect is stronger on AsCpf1, explaining its lower efficiency in ectothermic organisms. We capitalize on this property to show that temporal control of the temperature allows post-translational modulation of Cpf1-mediated genome editing. Finally, we determine that LbCpf1 significantly increases homology-directed repair in zebrafish, improving current approaches for targeted DNA integration in the genome. Together, we provide a molecular understanding of Cpf1 activity in vivo and establish Cpf1 as an efficient and inducible genome engineering tool across ectothermic species.

  • References31
  • Citations55

References

  • References31
  • Citations55

Mentioned in this Paper

In Vivo
Bacterial Proteins
Ribonucleoprotein Activity
Genome
CRISPR-Cas Systems
Gene Editing
Endonuclease
Complex (molecular entity)
Mediator of activation protein
Zebrafish

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