CRISPR/Cas13a Powered Portable Electrochemiluminescence Chip for Ultrasensitive and Specific MiRNA Detection

Advanced Science
Ting ZhouDa Xing

Abstract

MicroRNAs (miRNAs) have been widely investigated as potential biomarkers for early clinical diagnosis of cancer. Developing an miRNA detection platform with high specificity, sensitivity, and exploitability is always necessary. Electrochemiluminescence (ECL) is an electrogenerated chemiluminescence technology that greatly decreases background noise and improves detection sensitivity. The development of a paper-based ECL biosensor further makes ECL suitable for point-of-care detection. Recently, clustered regularly interspaced short palindromic repeats (CRISPR)/Cas13a as high-fidelity, efficient, and programmable CRISPR RNA (crRNA) guided RNase has brought a next-generation biosensing technology. However, existing CRISPR/Cas13a based detection often faces a trade-off between sensitivity and specificity. In this research, a CRISPR/Cas13a powered portable ECL chip (PECL-CRISPR) is constructed. Wherein target miRNA activates Cas13a to cleave a well-designed preprimer, and triggers the subsequent exponential amplification and ECL detection. Under optimized conditions, a limit-of-detection of 1 × 10-15 m for miR-17 is achieved. Through rationally designing the crRNA, the platform can provide single nucleotide resolution to dramatical...Continue Reading

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Citations

Jan 8, 2021·Biosensors & Bioelectronics·Peng-Fei LiuGuo-Xi Liang
Apr 22, 2021·Lab on a Chip·Wen-Chin LeeLung-Ming Fu
May 13, 2021·Analytical Chemistry·Ashwin Ramachandran, Juan G Santiago
Jun 29, 2021·Chemical Communications : Chem Comm·Jiali ZhaoZhou Nie
Jul 18, 2021·Nature Biomedical Engineering·Michael M KaminskiJames J Collins
Aug 7, 2021·Nature Chemical Biology·Tina Y LiuJennifer A Doudna
Aug 19, 2021·Analytical Chemistry·Panpan GaiFeng Li
Aug 25, 2021·Analytical Chemistry·Shaohua GongBo Tang
Sep 24, 2021·Wiley Interdisciplinary Reviews. RNA·Martyna KordyśZbigniew Warkocki
Oct 7, 2021·Chemical Society Reviews·Yanan TangX Chris Le

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Methods Mentioned

BETA
biosensing
nucleic acid amplification
biosensor
chip
electrophoresis
fluorescence assay
PCR
Assay

Software Mentioned

CAS
EXPAR
CFX Connect

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