CRISPR/Cas9-Mediated BRCA1 Knockdown Adipose Stem Cells Promote Breast Cancer Progression

Plastic and Reconstructive Surgery
Ruya ZhaoScott T Hollenbeck

Abstract

The tumor microenvironment within the breast is rich in adipose elements. The interaction between adipose cells and breast cancer is poorly understood, particularly as it pertains to patients with genetic susceptibility to breast cancer. This study focuses on the phenotype of human adipose-derived stem cells with the BRCA1 mutation and the effect they may have on breast cancer cell behavior. CRISPR/Cas9 was used to generate de novo BRCA1-knockdown human adipose-derived stem cells. The effect of the BRCA1 knockdown on the adipose-derived stem cell phenotype was compared to wild-type adipose-derived stem cells and patient-derived breast adipose-derived stem cells with known BRCA1 mutations. Interactions between adipose-derived stem cells and the MDA-MB-231 breast cancer cell line were evaluated. BRCA1-knockdown adipose-derived stem cells stimulated MDA-MB-231 proliferation (1.4-fold increase on day 4; p = 0.0074) and invasion (2.3-fold increase on day 2; p = 0.0171) compared to wild-type cells. Immunofluorescence staining revealed higher levels of phosphorylated ataxia telangiectasia-mutated activation in BRCA1-knockdown cells (72.9 ± 5.32 percent versus 42.9 ± 4.97 percent; p = 0.0147), indicating higher levels of DNA damage. Be...Continue Reading

References

Sep 20, 2005·Breast Cancer Research : BCR·Joanne KotsopoulosSteven A Narod
Aug 31, 2006·American Journal of Physiology. Endocrinology and Metabolism·Sagar GhoshRong Li
Jun 5, 2008·Stem Cells and Development·Ji Min YuJin Sup Jung
Feb 3, 2009·Carcinogenesis·Fabian L MuehlbergEckhard U Alt
Aug 18, 2009·The Journal of Urology·David S FinleyDavid K Ornstein
Aug 27, 2009·Cancer Research·Charis EngMichael C Ostrowski
Jan 19, 2010·Annual Review of Pathology·Jean-Philippe CoppéJudith Campisi
Jan 5, 2011·Protein & Cell·Jiaxue WuXiaochun Yu
Sep 2, 2011·Molecular Carcinogenesis·Gabriel WelteYao-Hua Song
Oct 12, 2011·Proceedings of the National Academy of Sciences of the United States of America·Hiroyuki KonishiBen Ho Park
Nov 1, 2011·International Journal of Cancer. Journal International Du Cancer·Eswaran DevarajanEckhard Alt
Nov 2, 2012·Cancer Metastasis Reviews·Yan MaoJianhua Wang
Dec 12, 2012·Toxicology and Applied Pharmacology·Ian W H JarvisKristian Dreij
Jan 5, 2013·Science·Le CongFeng Zhang
Sep 3, 2013·Journal of Graduate Medical Education·Gail M Sullivan, Richard Feinn
Oct 26, 2013·Nature Protocols·F Ann RanFeng Zhang
Apr 18, 2014·North American Journal of Medical Sciences·Zhi-Qiang LiuPing-Chang Yang
May 28, 2014·Nucleic Acids Research·Tessa G MontagueEivind Valen
Feb 19, 2015·Breast Cancer Research and Treatment·Mohammed AleskandaranyAndrew R Green
May 18, 2016·Nucleic Acids Research·Kornel LabunEivind Valen
Apr 30, 2017·Experimental and Therapeutic Medicine·Jian-Mei WangLi-Rong Yin
Dec 29, 2017·Advances in Experimental Medicine and Biology·Hitomi Sumiyoshi Okuma, Kan Yonemori

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Citations

Mar 1, 2019·Plastic and Reconstructive Surgery·Rachel A Sabol, Bruce A Bunnell
Aug 28, 2020·Frontiers in Cell and Developmental Biology·Wenjian ChenJun Sun
Feb 6, 2021·Cancers·Svetlana MiklikovaMarina Cihova
Jul 7, 2021·Journal of Mammary Gland Biology and Neoplasia·Adelina PlanggerGerhard Hamilton

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