CRISPR/Cascade 9-Mediated Genome Editing-Challenges and Opportunities

Frontiers in Genetics
Bhaskar RoyKarsten Kristiansen

Abstract

Clustered Regularly Interspaced Palindromic Repeats (CRISPR) and Cascade 9 (also known as Cas9, CRISPR associated protein 9) confer protection against invading viruses or plasmids. The CRISPR/Cascade 9 system constitutes one of the most powerful genome technologies available to researchers today. So far, this technology has enabled efficient genome editing and modification in several model organisms and has successfully been used in biomedicine and biomedical engineering. However, challenges for efficient and safe genetic manipulation in several organisms persist. Here, we review functional approaches and future challenges associated with the use of the CRISPR/Cascade 9 genome editing system and discuss opportunities, ethical issues and future directions within this field.

References

Sep 8, 1995·Science·R KühnK Rajewsky
Feb 1, 2006·The Journal of Biological Chemistry·Long ZhangYe-Guang Chen
Nov 9, 2006·Nucleic Acids Research·T Ryan GregoryMichael D Bennett
Sep 1, 2007·Nature Cell Biology·Nadia Rosenthal, Steve Brown
Jan 5, 2013·Science·Le CongFeng Zhang
Jan 31, 2013·Nature Biotechnology·Seung Woo ChoJin-Soo Kim
Jul 6, 2013·Cell Reports·Andrew R BassettJi-Long Liu
Jul 23, 2013·Nature Biotechnology·Patrick D HsuFeng Zhang
Oct 30, 2013·Cell Research·Yueqiang WangAnjiang Tan
Dec 4, 2013·Cell Research·Yuanwu MaLianfeng Zhang
Dec 18, 2013·Development, Growth & Differentiation·Hongmei Lisa LiAkitsu Hotta
Dec 18, 2013·Science·Ophir ShalemFeng Zhang
Feb 5, 2014·Biochemical and Biophysical Research Communications·Wataru FujiiKunihiko Naito
Mar 1, 2014·Methods : a Companion to Methods in Enzymology·Andrew Bassett, Ji-Long Liu
Mar 25, 2014·G3 : Genes - Genomes - Genetics·Zhaoyu XueGuanjun Gao
Apr 1, 2014·Nature Biotechnology·Hao YinDaniel G Anderson
Apr 5, 2014·Insect Biochemistry and Molecular Biology·Yuanyuan LiuQingyou Xia
Apr 29, 2014·Nature Biotechnology·Shengdar Q TsaiJ Keith Joung
Jul 9, 2014·Proceedings of the National Academy of Sciences of the United States of America·Fillip PortSimon L Bullock
Aug 28, 2014·FEBS Open Bio·Angus Yiu-Fai Lee, Kevin C Kent Lloyd
Sep 30, 2014·Cell·Randall J PlattFeng Zhang
Oct 23, 2014·Development·Ying PengStephen C Ekker
Nov 14, 2014·Plant & Cell Physiology·Masaki EndoSeiichi Toki
Nov 21, 2014·Development·Uwe IrionChristiane Nüsslein-Volhard
Mar 20, 2015·Nature·David Cyranoski
Mar 27, 2015·Nature·Edward LanphierJoanna Smolenski

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Citations

Nov 7, 2019·BioMed Research International·Odatha W KotagamaThelma Abeysinghe
Apr 10, 2020·Plant Biotechnology Journal·Debin ZhangFang Ding
Feb 8, 2019·Indian Journal of Clinical Biochemistry : IJCB·Rama Devi Mittal
Apr 26, 2019·Journal of Animal Science and Biotechnology·Guoyao Wu, Fuller W Bazer
Dec 7, 2018·Genes, Chromosomes & Cancer·Marion Cremer, Thomas Cremer
Sep 12, 2018·Cells·Mohamed A ElmonemElena N Levtchenko
Dec 4, 2020·Nucleic Acids Research·Amer EliasMikhail Kolot
Mar 9, 2021·Frontiers in Genetics·Collins N Khwatenge, Samuel N Nahashon
May 22, 2019·Seminars in Cell & Developmental Biology·Desh Deepak SinghSubhash K Tripathi
Mar 29, 2021·Molecular Biotechnology·Rajveer SinghDipanjan Ghosh
Jun 1, 2021·Molecular Biotechnology·Seyed Ahmad Rasoulinejad, Faezeh Maroufi
Jun 4, 2021·Frontiers in Molecular Biosciences·Susanne HoltzeArne Sahm
Aug 28, 2021·International Journal of Molecular Sciences·Wei Sheng SiewWei Hsum Yap

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Methods Mentioned

BETA
PMA
gene knockout
gene knock-in
gene knockdown
gene knock-down
gene knock-out
transfection

Software Mentioned

Cascade

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