CRISPR/dCas9-mediated inhibition of gene expression in Staphylococcus aureus

Journal of Microbiological Methods
Xiaoyun DongDacheng Wang

Abstract

The understanding of the genetic mechanism of Staphylococcus aureus requires efficient tools, however, genetic manipulation in S. aureus is always laborious and time-consuming. Here we proposed a novel CRISPR/dCas9 interference method for the rapid knockdown of target genes. Furthermore, multiple genes can be repressed simultaneously by using this method.

Citations

Nov 6, 2017·Archives of Gynecology and Obstetrics·C KouskoutiF Kainer
Aug 5, 2019·Methods : a Companion to Methods in Enzymology·Florence Depardieu, David Bikard
Jun 22, 2021·Access Microbiology·Ian R Monk, Timothy P Stinear
Jan 12, 2021·Briefings in Bioinformatics·Hui PengJinyan Li
Dec 22, 2021·Journal of Cellular and Molecular Medicine·Yingli JinLin Wang

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