PMID: 15240740Jul 9, 2004Paper

Crisscross CTL induction by SYT-SSX junction peptide and its HLA-A*2402 anchor substitute

The Journal of Immunology : Official Journal of the American Association of Immunologists
Kazunori IdaNoriyuki Sato

Abstract

To investigate the effects of anchor substitutions in SYT-SSX junction peptide, an HLA-A24 anchor residue (position 9) of the SYT-SSX B peptide (GYDQIMPKK) was substituted to more favorable residues according to the HLA-A24-binding motif. Among four substitutes constructed, a substitute with isoleucine (termed K9I peptide) most apparently enhanced the affinity for HLA-A24 molecule. Subsequent in vitro CTL induction analysis using PBMCs of 15 HLA-A24(+) synovial sarcoma patients revealed that the original B peptide allowed to induce synovial sarcoma-specific CTLs from 7 patients (47%), whereas such CTLs were inducible from 12 patients (80%) with K9I peptide. Moreover, the extent of cytotoxicity against HLA-A24(+) synovial sarcoma cell lines was higher in K9I peptide-induced CTLs than B peptide-induced CTLs. Influence of anchor substitution on peptide/TCR interaction was evaluated by cytotoxicity assays against autologous cells and tetramer analysis. CTLs induced from a synovial sarcoma patient using K9I peptide did not lyse autologous PHA blasts or EBV-infected B cells. In vitro stimulations of PBMCs from 5 HLA-A24(+) synovial sarcoma patients with K9I peptide increased the frequency of T cells reacting with both HLA-A24/K9I pep...Continue Reading

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Citations

Jan 20, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Tomohide TsukaharaToshihiko Yamashita
Sep 14, 2007·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Shigeharu KimuraToshihiko Yamashita
Dec 21, 2012·Expert Review of Vaccines·Jordan E BloomBrian M Olson
Mar 11, 2011·Journal of Immunoassay & Immunochemistry·Munehide NakatsugawaNoriyuki Sato
Feb 19, 2011·Sarcoma·Seth M PollackRobin L Jones
May 10, 2016·Expert Opinion on Biological Therapy·Tomohide TsukaharaToshihiko Torigoe
Apr 9, 2009·Pathology International·Noriyuki SatoToshihiko Torigoe
Aug 4, 2016·Immunotherapy·Matthew DallosSandra P D'Angelo
Jul 21, 2016·Pathology International·Tomohide TsukaharaToshihiko Torigoe
Aug 10, 2005·Journal of Translational Medicine·Satoshi KawaguchiToshihiko Yamashita
Jun 16, 2009·Journal of Translational Medicine·Tomohide TsukaharaNoriyuki Sato

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