Criteria and methodology for identifying respiratory denitrifiers.

Applied and Environmental Microbiology
I Mahne, J M Tiedje

Abstract

Respiratory denitrification is not always adequately established when bacteria are characterized. We have tested a simple method that allows one to evaluate whether the two necessary criteria to claim denitrification have been met, namely, that N(inf2) or N(inf2)O is produced from nitrate or nitrite and that this reduction is coupled to a growth yield increase. Microorganisms were cultured in sealed tubes under a helium headspace and in the presence of 0, 2, 4, 7, and 10 mM nitrate or nitrite. After growth had ceased, N(inf2) and N(inf2)O were quantified by gas chromatography and the final protein concentration was measured. Net protein production was linearly related to nitrate concentration for all denitrifiers tested and ranged from 2 to 6 g of protein per mol of electron equivalent reduced. Nitrogen recovery as N(inf2) plus N(inf2)O from nitrate and nitrite transformed exceeded 80% for all denitrifiers. We also suggest that a rate of N gas production of >10 (mu)mol/min/g of protein can be used as an additional characteristic definitive of denitrification since this process produces gas more rapidly than other processes. These characteristics were established after evaluation of a variety of well-characterized respiratory de...Continue Reading

References

Nov 1, 1977·Applied and Environmental Microbiology·D L EskewI P Ting
Mar 1, 1977·Bacteriological Reviews·R K ThauerK Decker
Apr 1, 1977·Applied and Environmental Microbiology·T N GambleJ M Tiedje
Jan 1, 1992·Applied and Environmental Microbiology·G B Smith, J M Tiedje
Jul 1, 1974·Canadian Journal of Microbiology·D G Patriquin, R Knowles
Apr 1, 1983·Applied and Environmental Microbiology·C A Carlson, J L Ingraham
Apr 1, 1994·Applied and Environmental Microbiology·R W YeJ M Tiedje
Jan 1, 1993·Archives of Microbiology·W G Zumft
Jan 1, 1988·Annual Review of Microbiology·L I Hochstein, G A Tomlinson
Dec 1, 1982·Applied and Environmental Microbiology·B H Bleakley, J M Tiedje

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Citations

Apr 20, 2001·FEMS Microbiology Ecology·C EtchebehereL Muxí
Nov 14, 2006·Journal of Bacteriology·Claribel Cruz-GarcíaJames M Tiedje
May 1, 2002·FEMS Microbiology Ecology·C EtchebehereL Muxí
May 12, 2010·FEMS Microbiology Ecology·Gesche BrakerRalf Conrad
Nov 15, 2012·Proceedings of the National Academy of Sciences of the United States of America·Robert A SanfordFrank E Löffler
Mar 19, 2015·Frontiers in Microbiology·Melike BalkHendrikus J Laanbroek
May 7, 2004·Applied and Environmental Microbiology·Reiko Sameshima-SaitoKiwamu Minamisawa
Sep 24, 2004·Canadian Journal of Microbiology·D ChènebyL Philippot
May 13, 2006·FEMS Microbiology Ecology·Mary Joyce Dinglasan-PanlilioElizabeth Edwards
Dec 14, 2005·Environmental Microbiology·Karrie A WeberEric E Roden
Mar 7, 2003·Canadian Journal of Microbiology·David M StamperOlli H Tuovinen
Mar 28, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Lars R BakkenAsa Frostegård
Apr 22, 2015·Scientific Reports·Koki MaedaLaurent Philippot
Jan 21, 2015·Journal of Environmental Quality·Kristopher S GuentzelJessica L Kozarek
Jan 27, 2011·Biochemical Society Transactions·Ines VerbaendertKim Heylen
Dec 6, 2011·International Journal of Systematic and Evolutionary Microbiology·Om PrakashJoel E Kostka
May 22, 2019·Scientific Reports·Alla Usyskin-TonneDror Minz
Nov 16, 2010·Applied and Environmental Microbiology·Satoshi IshiiKeishi Senoo
Jan 6, 2018·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Bo-Min KiKyung-Suk Cho
Oct 20, 2005·Journal of Bioscience and Bioengineering·Naoki Takaya
Jul 1, 1997·Applied and Environmental Microbiology·B Krause, K H Nealson
Aug 3, 2010·Systematic and Applied Microbiology·Silke FalkGesche Braker

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