Criterion values for multiplex SNP genotyping by the invader assay

Forensic Science International. Genetics
Hiroaki NakaharaKentaro Kasai

Abstract

We have developed a multiplex, single nucleotide polymorphism (SNP) typing system for forensic identification, based on the Invader assay. Using this system, fluorescence data for 21 SNP loci were collected and analyzed. We used endpoint genotyping, commonly used with the Invader assay, and also developed more reliable typing criteria because endpoint typing was occasionally unable to clearly identify the genotype of some loci. Analysis of the fluorescence data identified criteria that had high reproducibility for each genotype. One such criterion is the climbing angle of the curve resulting from two-dimensional plots of the two kinds of fluorescence used for the Invader assay. The climbing angle was observed at the time during the reaction when either or both of the fluorescence intensities increased most significantly. The angles were remarkably associated with homozygous genotypes. On the other hand, all heterozygote endpoint fluorescence ratios were highly reproducible and had very little aberration. These values enabled SNP typing to be more clearly defined compared with typing using only the endpoint fluorescence ratio, which is commonly used with the Invader assay. The values suggested in this study are easily calculated...Continue Reading

References

Mar 4, 2000·Biochimica Et Biophysica Acta·K NakamuraA Takasaki
Mar 21, 2001·Pharmacogenomics·L ForsR W Kwiatkowski
Aug 15, 2001·Journal of Human Genetics·Y OhnishiY Nakamura
Sep 26, 2002·Expert Review of Molecular Diagnostics·Monika de ArrudaEric B Rasmussen
Apr 15, 2005·Mutation Research·Michael Olivier
Apr 20, 2005·Forensic Science International : Synergy·António Amorim, Luísa Pereira
Nov 8, 2005·The Tohoku Journal of Experimental Medicine·Masahiro HiratsukaMichinao Mizugaki
Mar 3, 2007·Human Genetics·Andrew J PakstisKenneth K Kidd
Mar 13, 2009·Legal Medicine·Hiroaki NakaharaKazushige Kai

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Citations

Jun 29, 2015·Legal Medicine·Nakahara HiroakiSaito Kazuyuki
May 3, 2011·Analytical Chemistry·T A BrettellJ R Almirall

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