PMID: 7024219Feb 1, 1981Paper

Critical appraisal of complement dependent microlymphocytotoxicity assay for detecting donor-specific alloantibody pretransplant--importance of indirect immunofluorescence as a superior alternative

Human Immunology
P I LoboG Pirsch

Abstract

The ability of complement-dependent microlymphocytotoxicity assay (CdL) to detect and discriminate between the various types of donor-specific alloantibodies was reevaluated. Data obtained with the CdL assay on purified B and T lymphocytes at warm and cold temperatures was compared to other modes of antibody-detection, i.e., indirect immunofluorescence (IF) and the noncomplement-dependent antibody-dependent cellular cytotoxicity (ADCC). Additionally, the significance of antibodies as detected by CdL and IF was ascertained by correlating with kidney transplant outcome. It became apparent that the CdL assay identified weakly reactive HLA-ABC alloantibodies as being B cell specific. Such weakly reactive HLA-ABC antibodies were also not appreciated in the presence of the cold reactive IgM antibody. Accelerated rejections were the rule in the presence of weakly reactive HLA-ABC alloantibodies indicating that their detection was highly important. The IF assay could discriminate between the antibody class, could detect weakly reactive HLA-ABC alloantibodies, and could detect noncomplement fixing antibodies (ADCC). Further, use of IF prevented us from unnecessarily denying transplants to certain recipients when a positive CdL assay res...Continue Reading

References

Apr 26, 1975·Lancet·C R StillerA C Wallace
Dec 1, 1975·The Journal of Clinical Investigation·D A Horwitz, P I Lobo
May 1, 1978·Transplantation·V LepageJ Dausset
Aug 1, 1978·Transplantation·P I LoboL Rudolf
Aug 5, 1976·The New England Journal of Medicine·R B EttengerR N Fine
Apr 1, 1976·Transplantation·D E CrossF C Whittier
Dec 5, 1970·Lancet
Mar 1, 1969·Transplantation·D B AmosA Tiilikainen
Dec 5, 1964·Nature·P I TERASAKI, J D MCCLELLAND

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Citations

Jan 1, 1995·Transplant International : Official Journal of the European Society for Organ Transplantation·P I LoboT L Pruett
Sep 16, 2000·Transfusion·W M MontgomeryE Olivares
Jan 1, 1997·Transplant International : Official Journal of the European Society for Organ Transplantation·P I LoboC McCullough
Oct 1, 1990·Transfusion Medicine Reviews·A E von dem BorneR W Kuijpers
Mar 7, 2019·HLA : Immune Response Genetics·Daniel S RamonLeo Li-Ying Chan

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