Critical assessment of the nuclear import of plasmid during cationic lipid-mediated gene transfer

The Journal of Gene Medicine
V EscriouD Scherman

Abstract

Cationic lipid-mediated gene transfer is a promising approach for gene therapy. However, despite the significant amount of lipoplexes internalized by target cells, transgene expression remains too low. Obstacles to nuclear accumulation of plasmid DNA include: the passage of DNA across the cellular membrane, the dismantling of nucleolipidic particles in the cytoplasm and the nuclear import of plasmid DNA. The purpose of the present study was to evaluate the impact of cell status on cationic lipid-mediated transfer. Cells were either growth-arrested (by aphidicolin) or synchronized (by a classical double-thymidine block protocol) and cationic lipid-mediated transfection of these cells was evaluated. For the study of the nuclear import of plasmid DNA, two techniques were developed: microinjection of plasmid DNA into intact cells, and the use of cells permeabilized with digitonin. When CV-1 cells were growth-arrested by aphidicolin, cationic lipid-mediated gene transfer was inhibited. Hela cells were synchronized and incubated with lipoplexes at different times after release of the block. Gene expression was greatly enhanced when cells underwent mitosis. When transfection was performed during the early period after block release, w...Continue Reading

References

Aug 11, 1995·The Journal of Biological Chemistry·J ZabnerM J Welsh
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·M E DowtyJ A Wolff
May 4, 1995·Biochimica Et Biophysica Acta·H FarhoodL Huang
Feb 3, 1998·Nature Biotechnology·M G SebestyénJ A Wolff
Apr 29, 1998·The Journal of Biological Chemistry·H PollardD Escande
Jul 10, 1998·Virology·G R Whittaker, A Helenius
Jan 6, 1999·Proceedings of the National Academy of Sciences of the United States of America·M A ZantaJ P Behr
Apr 21, 1999·Biochimica Et Biophysica Acta·W C TsengT D Giorgio
Jul 8, 1999·Current Opinion in Cell Biology·S A Adam
Jul 8, 1999·Current Opinion in Cell Biology·D StofflerU Aebi
Sep 1, 1999·Gene Therapy·F SoubrierJ Crouzet
Nov 24, 1999·Human Gene Therapy·M BrissonL Huang
Jan 14, 2000·Molecular Biology Reports·S MarenziP Caiafa
Feb 17, 2000·Trends in Biotechnology·A Mountain
Feb 19, 2000·Gene Therapy·S Li, L Huang
Aug 10, 2000·Molecular Therapy : the Journal of the American Society of Gene Therapy·M B James, T D Giorgio

❮ Previous
Next ❯

Citations

Sep 12, 2002·Journal of Physiology and Biochemistry·A RochaJ M Coll
Nov 13, 2008·Cytotechnology·Hannes ReisingerRenate Kunert
Mar 10, 2006·Pharmaceutical Research·Marieke A E M van der AaDaan J A Crommelin
Jan 17, 2004·International Journal of Pharmaceutics·Rieko TachibanaHiroshi Kiwada
Feb 5, 2003·Advanced Drug Delivery Reviews·Virginie EscriouPierre Wils
May 22, 2002·Biochimica Et Biophysica Acta·Lina PrasmickaiteKristian Berg
Sep 3, 2002·International Journal of Pharmaceutics·Swayam PrabhaVinod Labhasetwar
Apr 16, 2002·Current Opinion in Biotechnology·Mark E Davis
Sep 1, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Gaëlle VandermeulenVéronique Préat
Jul 31, 2009·Chemical Communications : Chem Comm·Santanu Bhattacharya, Avinash Bajaj
Sep 2, 2008·Proceedings of the National Academy of Sciences of the United States of America·Joanna Y ChinPeter M Glazer
Dec 8, 2009·Biochemistry. Biokhimii︠a︡·Y S Tarahovsky
May 19, 2009·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Linfeng F LiYuehui H Ma
Nov 2, 2006·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Hidetaka AkitaHideyoshi Harashima
Nov 2, 2011·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Hidetaka Akita
Mar 17, 2010·The Journal of Reproduction and Development·Ying WangYue hui Ma
Mar 7, 2006·The Journal of Reproduction and Development·Jian-Ning YuJing-He Tan
Dec 24, 2005·Expert Opinion on Drug Delivery·Alain Rolland
Jan 3, 2012·Advanced Drug Delivery Reviews·Nathalie SymensKatrien Remaut
Oct 26, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Geertrui TavernierJoanna Rejman
Feb 5, 2010·Journal of Bioscience and Bioengineering·Kazumi HakamadaJun Miyake
Apr 28, 2009·Advanced Drug Delivery Reviews·Aaron M Miller, David A Dean
Jun 27, 2008·Tissue & Cell·Changqing LiuXuexi Tang
Oct 16, 2007·International Journal of Medical Microbiology : IJMM·Christoph SchoenJochen Stritzker
Oct 26, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Maayan Duvshani-Eshet, Marcelle Machluf
Aug 20, 2005·Journal of Cellular Physiology·Lilian KastrupVictor Shahin
May 11, 2006·The Journal of Gene Medicine·Stéphanie GrosseIsabelle Fajac
Apr 3, 2007·The Journal of Gene Medicine·Elizabeth CollinsMark Gumbleton
Jul 7, 2011·Animal Science Journal = Nihon Chikusan Gakkaihō·Chang-Qing LiuYue-Hui Ma
Nov 26, 2009·The Journal of Gene Medicine·Marie BretonNathalie Mignet
May 15, 2002·Biochimica Et Biophysica Acta·Muriel GolzioMarie-Pierre Rols
May 4, 2004·Ultrasound in Medicine & Biology·Vladimir G Zarnitsyn, Mark R Prausnitz
Oct 3, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Katrien RemautStefaan C De Smedt

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.