Critical conditions for separating the microheterogeneous components of glycoproteins by capillary electrophoresis

Journal of Chromatography. a
Y Chen

Abstract

To separate the microheterogeneous components of glycoproteins, capillary electrophoresis (CE) conditions were systematically investigated with ovalbumin from chicken egg white as the main testing sample. In addition to the well-known adsorption effect, a reversible capillary wall partitioning effect was found to contribute greatly to the separation and the favorable lubing wall was that coated with polyacrylamide. The separation media, especially the buffer composition, its concentration and pH, were also found to be critical. Buffers composed of boric acid and alcohol amines were demonstrated to be more effective media than gels and other solutions, but the selection of the buffer concentrations and pH was dependent on samples. By using free-solution CE with polyacrylamide-coated capillaries and boric acid-alcohol amine (BA) buffers, ovalbumin of agarose electrophoresis purity can be split into more than twenty peaks at pH 8.0 +/- 0.3 (0.2-0.4 mol/l BA). In contrast, free-solution CE with uncoated capillaries and gel electrophoresis with polyacrylamide-filled capillaries yielded only five or less peaks.

References

Feb 1, 1986·Archives of Biochemistry and Biophysics·Y KatoK Hotta
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·B T Sheares, P W Robbins
Jun 15, 1994·Analytical Chemistry·C A Monnig, R T Kennedy
Dec 1, 1957·Biochimica Et Biophysica Acta·L W CUNNINGHAMR B NUENKE

❮ Previous
Next ❯

Citations

Jul 20, 2007·Chemistry : a European Journal·Tao LiErkang Wang
Aug 19, 2008·The Analyst·Wei LiuMingdi Yan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.